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EXOTOXIN

Award Information

Agency:
Department of Health and Human Services
Branch:
N/A
Award ID:
4704
Program Year/Program:
1986 / SBIR
Agency Tracking Number:
4704
Solicitation Year:
N/A
Solicitation Topic Code:
N/A
Solicitation Number:
N/A
Small Business Information
Zymogenetics Inc
2121 North 35th Street Seattle, WA 98103
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Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No
 
Phase 1
Fiscal Year: 1986
Title: EXOTOXIN
Agency: HHS
Contract: N/A
Award Amount: $50,000.00
 

Abstract:

THE GOAL OF THIS PROJECT IS TO CREATE A STABLE MURINE MYELOMA CELL LINE THAT SECRETES A HUMAN ANTIBODY AGAINST ANTHRAX EXOTOXIN. THE MOLECULAR CLONING OF HUMAN IMMNUOGLOBULIN (IG) GENES FROM THE GENOMIC DNA OF A HUMAN/ MOUSE HYBRIDOMA IS PROPOSED. THE HUMAN IG GENES, WHICH ENCODE AN ANTIBODY AGAINST ANTHRAX EXOTOXIN, WILL BE SPLICEDTO PREVIOUSLY CHARACTERIZED MOUSE IG GENES. THE RESULTING CHIMERIC IG GENES CONTAINING MURINE TRANSCRIPTIONAL SIGNALS AND HUMAN IG CODING REGIONS WILL BE TRANSFECTED INTO A NON- PRODUCING MURINE MYELOMA CELL LINE. THE TRANSFECTED CELLS WILL THEN BE SCREENED FOR THOSE CELLS THAT PRODUCE HUMAN ANTIBODIES AGAINST ANTHRAX EXOTOXIN. CHARACTERIZATION AND SCALE UP OF THE CELL LINE CREATED IN PHASE I AS WELL AS THE ANTIANTHRAX ANTIBODY IT PRODUCES WILL RESULT IN A VALUABLE PRODUCT. FURTHERMORE, THE DATA OBTAINED SHOULD APPLY TO SUCCEEDING CELL LINES CREATED BY TRANSFECTING DIFFERENT IG GENES INTO THE SAME MURINE MYELOMA RECIPIENT. THIS TECHNOLOGY ALLOWS ONE TO EXPRESS AN ENDLESS SERIES OF DIFFERENT HUMAN ANTIBODIES IN THE SAME MURINE MYELOMA CELL LINE. FURTHERMORE, IT ALLOWS ONE TO PRODUCE NOVEL ANTIBODIES SUCH AS CHIMERIC MOUSE/HUMAN ANTIBODIES IN THE SAME MURINE MYELOMA CELL LINE.

Principal Investigator:

Wayne kindsvogel
PRINCIPAL INVESTIGATOR
2066324036

Business Contact:

Small Business Information at Submission:

Zymogenetics Inc
2121 North 35th Street Seattle, WA 98103

EIN/Tax ID:
DUNS: N/A
Number of Employees: N/A
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No