EXPRESSION OF HUMAN PROTEIN C IN MAMMALIAN CELLS

Award Information

Agency: Department of Health and Human Services	Branch: N/A	Award ID: 5009	Program Year/Program: 1987 / SBIR
Agency Tracking Number: 5009	Solicitation Year: N/A	Solicitation Topic Code: N/A	Solicitation Number: N/A

Small Business Information: Zymogenetics Inc
2121 North 35th Street Seattle, WA 98103
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Woman-Owned: No

Minority-Owned: No

HUBZone-Owned: No

Phase 2: Fiscal Year: 1987

Title: EXPRESSION OF HUMAN PROTEIN C IN MAMMALIAN CELLS

Agency: HHS

Contract: N/A

Award Amount: $279,000.00

Abstract:
A FULL-LENGTH CODING SEQUENCE FOR HUMAN PROTEIN C WILL BE CLONED. THIS WILL BE DONE EITHER BY OBTAINING A 5' END CLONE FROM A LIVER CDNA LIBRARY OR BY FUSING A GENOMIC DNA SEQUENCE TO CDNA, WHICH LACKS AN AMINOTERMINAL CODING SEQUENCE. ONCE THIS CLONE IS COMPLETE IS WILL BE INTRODUCEDINTO AN EXPRESSION VECTOR DESIGNED FOR HIGH-LEVEL EXPRESSIONIN MAMMALIAN CELLS. THIS CONSTRUCTION WILL BE TRANSFECTED INTO BHK CELLS AND EXPRESSED PROTEIN C ACTIVITY MEASURED. PROTEIN C WILL BE ASSAYED BY ELISA AND FOR ANTICOAGULANT ACTIVITY.

Principal Investigator:
Mark J Murray
Principal Investigator
2066324036

Business Contact:

Small Business Information at Submission:

Zymogenetics Inc.
2121 N 35th Street Seattle, WA 98103

EIN/Tax ID:

DUNS: N/A

Number of Employees: N/A

Woman-Owned: No

Minority-Owned: No

HUBZone-Owned: No