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CLONING, SEQUENCING, AND EXPRESSION OF HUMAN BSA LIPASE CDNA

Award Information

Agency:
Department of Health and Human Services
Branch:
N/A
Award ID:
7232
Program Year/Program:
1987 / SBIR
Agency Tracking Number:
7232
Solicitation Year:
N/A
Solicitation Topic Code:
N/A
Solicitation Number:
N/A
Small Business Information
Zymogenetics Inc
2121 North 35th Street Seattle, WA 98103
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Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No
 
Phase 1
Fiscal Year: 1987
Title: CLONING, SEQUENCING, AND EXPRESSION OF HUMAN BSA LIPASE CDNA
Agency: HHS
Contract: N/A
Award Amount: $50,000.00
 

Abstract:

BILE SALT-ACTIVATED LIPASE (BSAL) IS FOUND IN THE MILK OF A LIMITED NUMBER OF MAMMALS: HUMANS, GORILLAS, DOGS, AND CATS THE ENZYME PLAYS AN IMPORTANT ROLE IN THE INFANT DIET IN THEDIGESTION OF LIPIDS, CAUSING A STRIKING QUANTITATIVE DIF- FERENCE BETWEEN BREAST-FED AND FORMULA-FED INFANTS IN WEIGHTGAIN. IN THIS REGARD, IT IS IMPORTANT TO NOTE THAT BSAL IS ABSENT IN BOVINE MILK. IN PHASE I, ZYMOGENETICS, INC, PLANS TO CLONE, SEQUENCE, ANDEXPRESS THE CDNA FOR HUMAN BSAL. THIS WILL PROVIDE A FRAME WORK FOR PHASE II RESEARCH, WHEREIN RECOMBINANT HUMAN BSAL WILL BE PRODUCED IN LARGE QUANTITIES FOR SUPPLEMENTATION IN INFANT FORMULAS. EXPRESSION OF THE HUMAN BSAL CDNA WILL BE ACHIEVED BY CLONING INTO AN EXPRESSION VECTOR SUITABLE FOR PRODUCTION AT HIGH LEVELS IN SACCHAROMYCES CEREVISIAE. LEVELS OF EXPRESSION WILL BE ASCERTAINED BY ELISA AND DIRECTMEASUREMENTS OF ENZYMATIC ACTIVITY. THE RECOMBINANT PROTEINWILL BE PURIFIED AND CHARACTERIZED FOR ITS PHYSICAL AND CHEMICAL SIMILARITY TO THE PURIFIED HUMAN MILK ENZYME

Principal Investigator:

John m maraganore phd
2066324036

Business Contact:

Small Business Information at Submission:

Zymogenetics Inc
2121 North 35th Street Seattle, WA 98103

EIN/Tax ID:
DUNS: N/A
Number of Employees: N/A
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No