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LOW-COST HELPER-CELL MONITORING USING NOVEL CELL LINKERS

Award Information

Agency:
Department of Health and Human Services
Branch:
N/A
Award ID:
13659
Program Year/Program:
1990 / SBIR
Agency Tracking Number:
13659
Solicitation Year:
N/A
Solicitation Topic Code:
N/A
Solicitation Number:
N/A
Small Business Information
Zynaxis, Inc.
371 PHOENIXVILLE PIKE MALVERN, PA 19355
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Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No
 
Phase 1
Fiscal Year: 1990
Title: LOW-COST HELPER-CELL MONITORING USING NOVEL CELL LINKERS
Agency: HHS
Contract: N/A
Award Amount: $50,000.00
 

Abstract:

QUANTITATION OF CD4(+) LYMPHOCYTE LEVELS BY FLOW CYTOMETRY IS THE MOST COMMON MEASURE OF PROGRESSION OF HIV-INFECTED INDIVIDUALS TOWARD FRANK AIDS. THIS PROJECT WILL COUPLE NOVEL LIPOPHILIC CELL LINKERS WITH EXISTING MAGNETIC BEAD-BASED CELL SEPARATION TECHNIQUES TO PRODUCE AN INEXPENSIVE ALTERNATIVE SUITABLE FOR ROUTINE CD4(+) LYMPHOCYTE MONITORING, ALLOWING FCM RESOURCES TO BE DIRECTEDTOWARD OTHER TESTS REQUIRING MORE OPERATOR EXPERTISE. PRELIMINARY STUDIES USING FRESH MONONUCLEAR CELL PREPARATIONS FROM NORMAL INDIVIDUALS SHOW HIGH CORRELATION BETWEEN THIS ASSAY AND FLOW CYTOMETRY. PHASE I GOALS ARE DIRECTED TOWARD ASSAY SIMPLIFICATION AND VALIDATION ESSENTIAL TO DEVELOPMENT OF A CLINICALLY USEFUL IN VITRO DIAGNOSTIC TEST. GOALS INCLUDE: (1) DISCRIMINATION BETWEEN CD4(+) LYMPHOCYTES AND MONOCYTES; (2) SIMPLIFICATION OF SPECIMEN PREPARATION; (3) REDUCTION OF HIV-RELATED BIOHAZARDS; AND (4) EVALUATION OF THE RESULTING ASSAY IN WHOLE BLOOD SAMPLES HAVING A WIDE RANGE OF CD4(+) LYMPHOCYTELEVELS, OBTAINED FROM INDIVIDUALS AT DIFFERENT STAGES OF HIV INFECTION AND TREATMENT. A MEDICAL TECHNOLOGIST SHOULD BE ABLE TO IMPLEMENT THE FINAL PROCEDURE IN 1 TO 2 DAYS, MAKING IT SUITABLE FOR COMMUNITY HOSPITALS, LARGE CLINICS, AND THIRD-WORLD SETTINGS.

Principal Investigator:

Katharine Muirhead
2158892206

Business Contact:

Small Business Information at Submission:

Zynaxis Cell Science Inc
371 Phoenixville Pke Malvern, PA 19355

EIN/Tax ID:
DUNS: N/A
Number of Employees: N/A
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No