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MGB Eclipse Probe Detection of Category A Organisms

Award Information

Agency:
Department of Health and Human Services
Branch:
N/A
Award ID:
65933
Program Year/Program:
2003 / SBIR
Agency Tracking Number:
AI052905
Solicitation Year:
N/A
Solicitation Topic Code:
N/A
Solicitation Number:
N/A
Small Business Information
EPOCH BIOSCIENCES, INC.
EPOCH BIOSCIENCES, INC. 21720 23RD DR SE, #150 BOTHELL, WA 98021-3906
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Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No
 
Phase 1
Fiscal Year: 2003
Title: MGB Eclipse Probe Detection of Category A Organisms
Agency: HHS
Contract: 1R43AI052905-01A1
Award Amount: $100,000.00
 

Abstract:

DESCRIPTION (provided by investigator): Fluorogenic minor groove binder (MGB) probes containing an MGB-quencher at the 5'-end and a fluorophore at the 3'-end have been recently reported. These probes fluoresce on hybridization to the complementary targets. The 5'-MGB-quencher group prevents 5' - nuclease digestion by Taq polymerase during homogeneous amplification. The 5'-MGB-quencher-oligonucleotide-fluors can be used as probes in general nucleic acid and SNP detection assays. We propose to use a new software prediction program to develop MGB Eclipse probe assays to detect Clostridium botulinum, Bacillus anthracis and Yersinia pestis. The designed probes and primers will be optimized with use of three modified bases to improve probe performance of AT- and GC-rich sequences. In the Phase 1 effort, we will design and optimize the primers and probes and demonstrate function of the MGB Eclipse probe assays on a set of known target samples. The specificity of the probes will be evaluated against panels of closely related non-pathogenic organisms. In Phase II, we will expand the number of Category A organisms and improve throughput by multiplexing. In addition, commercial prototype MGB Eclipse(TM) probe assays will be developed for each of these organisms.

Principal Investigator:

Irina A. Afonina
4254825165
IAFONINA@EPOCHBIO.COM

Business Contact:

Walt Mahoney
4254825156
WMAHONEY@EPOCHBIO.COM
Small Business Information at Submission:

EPOCH BIOSCIENCES, INC.
EPOCH BIOSCIENCES, INC. 21720 23RD DR SE, #150 BOTHELL, WA 98021

EIN/Tax ID: 911311592
DUNS: N/A
Number of Employees: N/A
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No