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Nanofilament Silicon for Ultrasensitive Proteomics

Award Information

Agency:
Department of Health and Human Services
Branch:
N/A
Award ID:
89200
Program Year/Program:
2008 / SBIR
Agency Tracking Number:
GM084525
Solicitation Year:
N/A
Solicitation Topic Code:
N/A
Solicitation Number:
N/A
Small Business Information
IO BIOSYSTEMS, INC.
IO BIOSYSTEMS, INC. 5619 SONOMA RD BETHESDA, MD 20817
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Woman-Owned: Yes
Minority-Owned: No
HUBZone-Owned: No
 
Phase 1
Fiscal Year: 2008
Title: Nanofilament Silicon for Ultrasensitive Proteomics
Agency: HHS
Contract: 1R43GM084525-01
Award Amount: $400,002.00
 

Abstract:

DESCRIPTION (provided by applicant): A novel platform for ultrasensitive and matrix-free laser desorption/ionization mass spectrometry (LDI-MS) based on nanofilament silicon surfaces is proposed. Currently, the sensitivity and overall functionality of matr ix-free LDI-MS performed on porous silicon (pSi) chips are limited by a number of factors imposed by standard pSi substrates. In this proposal, we seek to address these issues through the development of novel silicon nanofilament substrates fabricated usin g metal-assisted oxidative etching and reactive-ion etching, with electrowetting used to provide dynamic control over surface hydrophobicity. This feature will allow switching of the surfaces from a superhydrophobic state to a strongly hydrophilic state, a llowing for complete wetting of the nanofilaments for optimal sample deposition while limiting sample dispersion and providing a method to achieve on-target sample concentration, thereby enabling routine detection limits of 10-100 zmol (10-20 to 10-19 mol) for complex peptide samples. PUBLIC HEALTH RELEVANCE: The analysis of proteins from biological samples is enabling advances in the treatment and diagnosis of disease. This project addresses the development of an ultrasensitive nanotechnology-based platform which can improve the ability to perform protein analysis from limited samples such as tissue biopsies.

Principal Investigator:

Business Contact:

Small Business Information at Submission:

IO BIOSYSTEMS, INC.
IO BIOSYSTEMS, INC. 5619 SONOMA RD BETHESDA, MD 20817

EIN/Tax ID: 126053175
DUNS: N/A
Number of Employees: N/A
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No