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Integrated Assembly Software for Sanger and Next Generation Sequence…

Award Information

Agency:
Department of Health and Human Services
Branch:
N/A
Award ID:
85741
Program Year/Program:
2007 / SBIR
Agency Tracking Number:
GM082117
Solicitation Year:
N/A
Solicitation Topic Code:
N/A
Solicitation Number:
N/A
Small Business Information
DNASTAR, INC.
3801 REGENT STREET MADISON, WI 53705-
View profile »
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No
 
Phase 1
Fiscal Year: 2007
Title: Integrated Assembly Software for Sanger and Next Generation Sequence Technologies
Agency: HHS
Contract: 1R43GM082117-01
Award Amount: $136,286.00
 

Abstract:

DESCRIPTION (provided by applicant): Obtaining complete genome DNA sequences of organisms from bacteria to man is a hallmark achievement of 20th century science and has had a huge impact on the biological sciences as well as the practice of medicine. Sequ ence assembly software played a critical role in making this possible. Now, there is a new rapid and dramatic fall in the cost of sequence data acquisition due to the emergence of revolutionary new sequencing machines that can turn out data in great quanti ties for a fraction of the cost. This is initiating a second wave of sequencing on a far greater scale and opens up many new applications, in addition to sequencing new genomes, that were heretofore not dreamed of or too costly to carry out. Projects such as using whole genome snapshots to identify crucial sequence changes that occur during evolution, metagenomic sequencing of communities, and medical diagnostic applications that can help track down alterations in genomic DNA related to disease are all be coming feasible. Medical scientists and entrepreneurs are even dreaming of the time when complete human genomes can be determined for about 1000, although this awaits yet another revolutionary advance in sequence data acquisition. Utilizing the present fl ood of new data will require corresponding progress in assembly software performance. We propose here to develop and evaluate prototype software approaches that can take advantage of all the new data gathering techniques. This will require developing new a lgorithms that take advantage of the abundant data generated to overcome bottlenecks that have previously limited the accuracy and completeness of assemblies necessitating expensive manual intervention to correct. As an independent software developer witho ut vested ties to particular technical approaches, DNASTAR is uniquely positioned to provide a trusted implementation that can effectively combine data from all approaches allowing researchers the freedom to use whatever combination of technologies that be st fits their needs. Our proposed new software techniques will make repeat handling, scaffold ordering and annotation far more efficient, and we predict the speed of assembly will also be increased dramatically. These feasibility experiments will be conduc ted on the strong foundation of DNASTAR's high performance SeqMan Genome Assembler platform. This will maximize the probability that a robust, commercially attractive product will emerge after Phase II with the high performance and accuracy needed by the l arge government sponsored sequencing centers, commercial and clinical sequencing operations as well as by individual research laboratories. We are embarking on a new era in medicine in which the genetic basis for diseases will be determined, both at the ge neral public level and as individuals. This is being made possible, in part, by dramatic improvements in the ability to decipher vast amounts of DNA at a greatly reduced cost. This proposal aims to develop the computational software needed to convert that mass of coded data into medically useful information.

Principal Investigator:

Timothy J. Durfee
6082373082
DURFEET@DNASTAR.COM

Business Contact:

Thomas Schwei
toms@dnastar.com
Small Business Information at Submission:

DNASTAR, INC.
DNASTAR, INC. 3801REGENT STREET MADISON, WI 53705

EIN/Tax ID: 139150045
DUNS: N/A
Number of Employees: N/A
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No