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CONSTRUCTION OF A MAMMALIAN EXPRESSION VECTOR FOR THE PRODUCTION OF IGF-1 AND…

Award Information

Agency:
National Science Foundation
Branch:
N/A
Award ID:
10738
Program Year/Program:
1989 / SBIR
Agency Tracking Number:
10738
Solicitation Year:
N/A
Solicitation Topic Code:
N/A
Solicitation Number:
N/A
Small Business Information
Incell Corp
9075 W Heather Ave P O Box 23767 Milwaukee, WI 53223
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Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No
 
Phase 1
Fiscal Year: 1989
Title: CONSTRUCTION OF A MAMMALIAN EXPRESSION VECTOR FOR THE PRODUCTION OF IGF-1 AND THE COORDINATED EXPRESSION OF BIOLOGICALLY ACTIVE HETERODIMERIC PROTEINS
Agency: NSF
Contract: N/A
Award Amount: $49,950.00
 

Abstract:

A NEED EXISTS FOR A SOURCE OF BIOLOGICALLY ACTIVE HUMAN PROTEINS, PARTICULARLY THOSE WITH COMPLEX TERTIARY OR QUATERNARY STRUCTURE WHICH REQUIRE MODIFICATION FOR FULL BIOLOGICAL ACTIVITY. CURRENT PRODUCTION OF RECOMBINANT MOLECULES BY BACTERIA HAS LIMITED ABILITY TO SATISFY THIS NEED. CULTURED MAMMALIAN CELLS PROVIDE GREATER PROMISE AS PRODUCTION SYSTEMS. CELL LINES, SUCH AS CHINESE HAMSTER OVARY (CHO), HAVE BEEN SHOWN CAPABLE OF EXPRESSING AND PROPERLY PROCESSING A VARIETY OF HUMAN RECOMBINANT PROTEINS,AND ARE ADAPTABLE TO LARGE-SCALE CULTIVATION IN SERUM FREE MEDIA. THIS PROPOSAL ADDRESSES THE NEED FOR AN IMPROVED EXPRESSION SYSTEMS TO FACILITATE THE PRODUCTION OF PROTEINS THAT ARE COMPOSED OF TWO DIFFERENT SUBUNITS. THE SYSTEM PROPOSED WILL UTILIZE A PLASMID VECTOR CONTAINING TRANSCRIPTION PROMOTERS FROM SV40 AND THE MOUSE MAMMARY TUMOR VIRUS, WHICH IS INTEGRATED INTO THE GENOME OF CHINESE HAMSTER OVARY CELLS (CHO). IN THIS CONSTRUCTION. BOTH PROMOTERS WILL BE CAPABLE OF DIRECTING HIGH LEVELS OF PRODUCTION, AND THE RATIO OF SYNTHESIS OF THE TWO PROTEINS PRODUCED CAN BE ADJUSTED TO ACHIEVE THE PROPER RATIO OF PRECURSOR PROTEINS FOR EFFICIENT FOLDING AND FINAL ASSEMBLY. THE PLASMID WILL BE CONSTRUCTED AND TESTED FOR EXPRESSION USING BACTERIAL REPORTER GENES. GROWTH AND EXPRESSION IN SERUM FREE MEDIA WILL BE OPTIMIZED IN THE PRODUCTION OF HUMAN INSULIN-LIKE GROWTH FACTOR-1 (IGF-1), A SMALL BIOLOGICALLY IMPORTANT PEPTIDE CURRENTLY PRODUCED BYTHIS LABORATORY USING A RECOMBINANT BACTERIAL SYSTEM.

Principal Investigator:

Gopalam Somasekhar
Principal Investigator
4143543311

Business Contact:

Small Business Information at Submission:

Incell Corp
9075 W Heather Ave P O Box 23767 Milwaukee, WI 53223

EIN/Tax ID:
DUNS: N/A
Number of Employees: N/A
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No