The eventual goal of the current research is to develop probes for in vivo imaging of gene expression and for in vivo targeting of cells for killing or remediation. The current proposal describes a novel nucleic acid probe design that will provide the basis of the new technology required for these… More

70846 Oilseed contains sterols and related compounds with economic potential. The extraction and analysis of these compounds would be aided by the availability of highly selective aptamers with high affinities for their particular sterol ligands. This project will develop aptamers for use in… More

DESCRIPTION (provided by applicant): We propose to develop a novel cancer therapy using allosteric reagents for concentrating a prodrug around a target cancer cell. Our aptamer-based design, called a CLAMP (cis-linked aptamers for medical or microanalytical procedures), is a nucleic acid with two… More

DESCRIPTION (provided by applicant): Detection and treatment of cancer can be greatly facilitated by noninvasive in vivo imaging techniques. In vivo imaging methods are most effective if they can be seamlessly tied to therapies. We propose to develop a novel imaging system using allosteric… More

DESCRIPTION (provided by applicant): Phase I The current proposal describes the selection of an aptamer that recognizes the drug PD173955, which is one of large family of substituted pyrido[2,3-d]pyrimidine inhibitors of tyrosine protein kinases. An aptamer is a single stranded nucleic acid that… More