USA flag logo/image

An Official Website of the United States Government

Targeted Aptamer Probes for Nucleic Acid Detection

Award Information

Agency:
Department of Health and Human Services
Branch:
N/A
Award ID:
54267
Program Year/Program:
2001 / STTR
Agency Tracking Number:
1R41AI049689-01
Solicitation Year:
N/A
Solicitation Topic Code:
N/A
Solicitation Number:
N/A
Small Business Information
MOLECULAR EXPRESS, INC.
1111 N HYLAND AVE AMES, IA 50014-
View profile »
Woman-Owned: Yes
Minority-Owned: No
HUBZone-Owned: No
 
Phase 1
Fiscal Year: 2001
Title: Targeted Aptamer Probes for Nucleic Acid Detection
Agency: HHS
Contract: PHS2001-2
Award Amount: $106,257.00
 

Abstract:

The eventual goal of the current research is to develop probes for in vivo imaging of gene expression and for in vivo targeting of cells for killing or remediation. The current proposal describes a novel nucleic acid probe design that will provide the basis of the new technology required for these applications. These probes are also expected to be a major improvement over current probes for applications such as the analysis of bacterial and other contamination of foods and in the analysis of specific gene expression in cell and tissue samples. We call these "Targeted Revealed Aptamer Probes" (TRAPS). The basic innovation in the proposed TRAP design is the inclusion of an attenuated aptamer that is revealed on hybridization with a target nucleic acid. In the current proposal, the following specific aims are proposed: * Produce a basic TRAP probe. Determine that the TRAP probe only binds its ligand when the probe is hybridized with complementary RNA. Test for proportionality between the amounts of complementary RNA and ligand binding to the probe. Test for specificity of the interaction between the TRAP and the target RNA. * Produce a capture TRAP probe that can be used in capture assays and test its efficacy. PROPOSED COMMERCIAL APPLICATIONS: Initially, these probes are expected to be a major improvement on the current nucleic acid probes used for detection of DNA and RNA sequences such as in the analysis of bacterial and other contamination of foods and in the analysis of specific gene expression in cell and tissue samples. After further development, the probes will be used for in vivo imaging of gene expression and for targeting cells in vivo so that they can be killed or remediated.

Principal Investigator:

Richard T. Hamilton
5152925141
MOLEXPRESS@ISUNET.NET

Business Contact:

Nilsen-hamilton, marit
5152925141
MOLEXPRESS@ISUNET.NET
Small Business Information at Submission:

MOLECULAR EXPRESS, INC.
1111 N HYLAND AVE AMES, IA 50014-4007 AMES, IA 50014

EIN/Tax ID: 421398933
DUNS: N/A
Number of Employees: N/A
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No
Research Institution Information:
IOWA STATE UNIVERSITY
IOWA STATE UNIVERSITY
AMES, IA 52307
RI Type: Nonprofit college or university