Receptor Assay by PCR to Detect IL 2 Responsive Lymphoma
1 R43 CA64030-1,
Resting normal cells do not express interleukin-2 receptor (IL-2R). The receptor is expressed bysome of the activated cells such as those in certain forms of lymphoid malignancies, autoimmunediseases, and graft rejection. Taking advantage of this property of IL-2R, several IL-2R directed clinicaltrials have been reported with variable successes. For example, IL-2 combined to diphtheria toxininduced significant tumor regression in patients with hematologic malignancies. However, in the past,investigators were unable to demonstrate the relationship between the level of IL-2R expression andtumor response primarily because of the inability to accurately measure the high affinity IL-2R levels.Monoclonal anti-Tac conjugated with a pseudomonas endotoxin or fusion toxin consisting of IL-2 andPE40 also have been developed to target IL-2 expressing cells. In addition, the monoclonal antibodiesprepared against IL-2 receptor induced remission in some patients with T-cell leukemia. These studiessupport the need for development of a rapid, sensitive, specific, and uncomplicated assay to measureIL-2R levels. Based on their experience with receptor transcript phenotyping (RTP) assay forerythropoietin receptor, the Investigators now propose to develop and assay based on PCR quantitationof IL-2R subunit messenger RNAs from peripheral blood mononuclear cells and sorted tumor biopsies.They will design and test PCR primers specific for IL-2Ra mRNA and determine the tissue specificity ofthe RTP assay by screening several cell lines. The quantitative aspect of the PCR will be tested first bynormalizing dilutions of samples with internal standard such as GAPDH gene. The Investigators,however, will attempt to develop absolute quantitation of IL-2a mRNA by template competition. Finally,binding assays will be performed to determine by Scatchard analysis the affinity of binding sites onselected cell lines.
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Principal Investigator:David Hankins
4853 Cordell Avenue #1407 Bethesda, MD 20814
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