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Double Capture Amplification System for Bacterial Pathogen Detection in

Award Information

Agency:
Department of Commerce
Branch:
N/A
Award ID:
43562
Program Year/Program:
1999 / SBIR
Agency Tracking Number:
43562
Solicitation Year:
N/A
Solicitation Topic Code:
N/A
Solicitation Number:
N/A
Small Business Information
PROED, INC.
9290 GAITHER RD GAITHERSBURG, MD 20877
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Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No
 
Phase 2
Fiscal Year: 1999
Title: Double Capture Amplification System for Bacterial Pathogen Detection in
Agency: DOC
Contract: N/A
Award Amount: $300,000.00
 

Abstract:

Not Available Bacterial pathogens in salmonids, such as Renibacterium salmoninarum and Aeromonas salmonicida, are difficult to identify by conventional diagnosis due to their extremely slow growth and the absence of an efficient selective medium. Diseases caused by these pathogens represent a serious problem both in the fishing industry and for conservation efforts. While several PCR-based assays appear to be useful, they are generally complex laboratory procedures and not suitable for large scale screening. We have proposed to develop a novel screening method, the ¿Double Capture Amplification System¿ (DCAS), to detect R. salmoninarum in salmonids. This procedure combines several powerful molecular biology tools in an innovative manner to develop a rapid, non-lethal, highly sensitive assay kit. Our Phase 1 research has demonstrated the feasibility of the proposed assay and initiated development of the prototype diagnostic kit. The major goals for the second phase of this project are to adapt, and optimize the assay for the detection of Renibacterium salmoninarum in various tissues in fish, determine the sensitivity and specificity of the assay, and begin production and quality control testing of the survey kits. In addition, we will initiate studies for the adaptation of DCAS to other organisms such as Aeromonas salmonicida.

Principal Investigator:

Business Contact:

Small Business Information at Submission:

Proed, Inc.
9290 Gaither Road Gaithersburg, MD 20877

EIN/Tax ID:
DUNS: N/A
Number of Employees: N/A
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No