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FP-Aptamer Reader for Algal Toxins

Award Information

Agency:
Department of Commerce
Branch:
N/A
Award ID:
57290
Program Year/Program:
2002 / SBIR
Agency Tracking Number:
2002-23
Solicitation Year:
N/A
Solicitation Topic Code:
N/A
Solicitation Number:
N/A
Small Business Information
OMNISITE BIODIAGNOSTICS, INC.
101 West Sixth Street Suite 200 Austin, TX 78701
View profile »
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No
 
Phase 1
Fiscal Year: 2002
Title: FP-Aptamer Reader for Algal Toxins
Agency: DOC
Contract: DG1330-02-CN-0039
Award Amount: $49,989.00
 

Abstract:

OmniSite BioDiagnostics (OmniSite) proposes to develop artificial receptors composed of DNA oligomers (called ¿Aptamers") for binding and detecting algal toxins. DNA aptamers can be mass produced with relative ease compared to antibody production and purification. The aptamers will be 5'-Cy 5 fluorochrome labeled and used in simple one-step binding fluorescence polarization (FP) assays that do not require wash steps. In Phase 1, OmniSite proposes to generate a set of specific fluoresceinated DNA aptamers to bind and detect Brevetoxin and Saxitoxin. Detection of the aptamer bound toxins will be accomplished via an FP breadboard system to be designed and constructed by OmniSite. In Phase 2, OmniSite will produce a hand-held FP prototype detector with wireless Internet connectivity so that test results can be accessed remotely. In addition, OmniSite will clone and sequence the anti-toxin aptamers and optimize FP aptamer assays for field use by water and seafood inspectors

Principal Investigator:

John G. Bruno
Chief Technical Officer
5124797732
bruno@spec.com

Business Contact:

James J. McNeal
Contracts Director
5124797732
mcneal@spec.com
Small Business Information at Submission:

OmniSite BioDiagnostics
101 West Sixth Street Suite 200 Austin, TX 78701

EIN/Tax ID: 742975605
DUNS: N/A
Number of Employees:
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No