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Handheld FP - Gene Microarray Reader for Assessment of Radiation Damage

Award Information

Agency:
National Aeronautics and Space Administration
Branch:
N/A
Award ID:
56652
Program Year/Program:
2002 / SBIR
Agency Tracking Number:
013793
Solicitation Year:
N/A
Solicitation Topic Code:
N/A
Solicitation Number:
N/A
Small Business Information
OMNISITE BIODIAGNOSTICS, INC.
101 West Sixth Street Suite 200 Austin, TX 78701
View profile »
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No
 
Phase 1
Fiscal Year: 2002
Title: Handheld FP - Gene Microarray Reader for Assessment of Radiation Damage
Agency: NASA
Contract: NAS3-02020
Award Amount: $69,995.00
 

Abstract:

Omnisite BioDiagnostics (OmniSite) proposes to develop a handheld fluorescence polarization (FP) reader for genetic microarrays to assess transcriptional changes in the genes of radiation-exposed astronauts. The reader will be capable of extracting messenger RNA (mRNA) from the blood or other tissues of an astronaut and looking for changes in the transcription of radiation-associated genes such as p53 on commercially available microscope slide-based gene arrays. Amplification of the RNA will be performed either by a variation of Rolling Circle Amplification (RCA) or Reverse Transcriptase-Polymerase Chain Reaction (RT-PCR). The advantage of using the FP mode of detection on the microarray is obviating the need for wash steps, thus making assays simpler and faster. FP enables homogenous one-step assays without sacrificing sensitivity.In Phase I, OmniSite will provide proof-of-concept for the detection of mRNAs on a gene array using an FP breadboard setup. OmniSite will also provide design parameters for a miniaturized handheld FP reader. In phase II, OmniSite will construct the handheld FP reader and demonstrate its use on real animal blood samples.

Principal Investigator:

John Bruno,
Principal Investigator
5124797732
bruno@spec.com

Business Contact:

Syd Ulvick,
CTO & VP Development
5124797732
ulvick@spec.com
Small Business Information at Submission:

OmniSite BioDiagnostics Inc.
101 W. Sixth Street, Suite 200 Austin, TX 78701

EIN/Tax ID: 742975605
DUNS: N/A
Number of Employees:
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No