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RECOMBINANT DNA-DERIVED PERTUSSIS SUBUNIT VACCINE

Award Information

Agency:
Department of Health and Human Services
Branch:
N/A
Award ID:
4684
Program Year/Program:
1988 / SBIR
Agency Tracking Number:
4684
Solicitation Year:
N/A
Solicitation Topic Code:
N/A
Solicitation Number:
N/A
Small Business Information
Amgen
1900 Oak Terrace Ln Thousand Oaks, CA 91320
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Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No
 
Phase 2
Fiscal Year: 1988
Title: RECOMBINANT DNA-DERIVED PERTUSSIS SUBUNIT VACCINE
Agency: HHS
Contract: N/A
Award Amount: $500,000.00
 

Abstract:

PERTUSSIS IS A DISEASE OF GLOBAL SIGNIFICANCE THAT PARTICULARLY AFFECTS INFANTS DURING THE FIRST YEAR OF LIFE. CURRENTLY LICENSED PERTUSSIS VACCINES ARE HIGHLY EFFICACIOUSBUT QUESTIONS REGARDING THEIR SAFETY HAVE LED TO PUBLIC FEARS OF VACCINE-RELATED INJURIES. THESE INVESTIGATIONS EXAMINE THE POSSIBILITY OF PRODUCING A SUBUNIT VACCINE COMPOSED OF PERTUSSIS ANTIGENS EXPRESSED IN HETEROLOGOUS HOSTS BY VIRTUE OF RECOMBINANT DNA METHODOLOGY. IN PRELIMINARY EXPERIMENTS, SPECIFIC ANTIGENIC COMPONENTS OF THE BACTERIUM BELIEVED CAPABLE OF ELICITING OR AUGMENTING PROTECTIVE IMMUNE RESPONSES AGAINST DISEASE WILL BE ISOLATED. THESE COMPONENTS ARE: (1) THE S-1 SUBUNIT OF THEPERTUSSIS TOXIN; (2) THE 58,000-DALTON SUBUNIT OF THE FILAMENTOUS HEMAGGLUTININ; AND (3) EACH OF THREE SEROTYPE- SPECIFIC FIMBRIAL AGGLUTINOGENS. N-TERMINAL AMINO ACID SEQUENCES WILL BE OBTAINED FOR EACH POLYPEPTIDE WITH A GAS-PHASE MICROSEQUENATOR. A SERIES OF OLIGONUCLEOTIDE PROBES REPRESENTING PREDICTED NUCLEOTIDE SEQUENCES ENCODING EACH ANTIGEN WILL BE SYNTHESIZED AND USED AS HYBRIDIZATION PROBES TO ISOLATE THE GENES FOR THESE PROTEINS FROM B. PERTUSSIS GENOMIC LIBRARIES. IN LATER INVESTIGATIONS, THESEGENES WILL BE INSTALLED IN HOST-VECTOR SYSTEMS CAPABLE OF HIGH-LEVEL EXPRESSION OF HETEROLOGOUS PROTEINS. THE RECOMBINANT-DERIVED PROTEINS SUBSEQUENTLY PRODUCED WILL BE ASSESSED FOR IMMUNOPOTENCY IN EXPERIMENTAL ANIMALS.

Principal Investigator:

W Neal Burnette
Principal Investigator
8054995725

Business Contact:

Small Business Information at Submission:

Amgen
1909 Oak Terrace Lane Thousand Oaks, CA 91320

EIN/Tax ID:
DUNS: N/A
Number of Employees: N/A
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No