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Novel HBV Therapeutic with Potential for Treatment of Drug-Resistant Infection

Award Information

Agency:
Department of Health and Human Services
Branch:
N/A
Award ID:
Program Year/Program:
2011 / SBIR
Agency Tracking Number:
R44AI077123
Solicitation Year:
2011
Solicitation Topic Code:
NIAID
Solicitation Number:
PA10-123
Small Business Information
PHARMABRIDGE, INC.
3805 OLD EASTON ROAD DOYLESTOWN, PA -
View profile »
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No
 
Phase 2
Fiscal Year: 2011
Title: Novel HBV Therapeutic with Potential for Treatment of Drug-Resistant Infection
Agency: HHS
Contract: 2R44AI077123-02A1
Award Amount: $3,000,000.00
 

Abstract:

DESCRIPTION (provided by applicant): This is a Phase II proposal to advance development of disubstituted tetrahydro-triazolo-pyrimidines (TTPs) as specific inhibitors of hepatitis B virus surface antigen (HBsAg) secretion in chronic infection. High serum levels of HBsAg (antigenemia) are believed to underlie immunotolerance toward the infection. Although they are clinically beneficial, the current small-molecule therapies rarely result in significant loss of serum HBsAg, elicit varying degrees of resistance, and are not curative. The parent compound of the TTPs was discovered in a high throughput screening campaign for HBsAg secretion inhibitors in a cell culture model. As proposed in our Phase I project, we 1) carried out extensive SAR studies to explore the salient features of the original structure, resulting in an increase in potency of more than 10-fold and improved chemical tractability; 2) confirmed the prediction that this compound family is active in inhibiting HBsAg secretion of HBV variants that are resistant to current small molecule drugs; and 3) identified formulation conditions for two lead compounds, improved solubility through salt formation, and characterized them in vivo for single dose ADMET properties. In Phase II of our project, we propose the following aims: 1) characterize the racemic properties of one lead compound, to determine whether enantiomeric separation will be necessary; 2) develop scale-up chemistry protocols amenable to eventual GMP manufacture, and produce sufficient non-GMPmaterial for Phase II in vivo studies; 3) carry out dose range-finding ADMET studies on the lead compounds; 4) determine efficacy of the lead compounds on antigenemia in mouse and woodchuck models of hepatitis B, with an eye on possible toxicity due to HBsAg retention in the liver; 5) assess the potential for synergy in combination studies with our compound and current antivirals in cell culture and in vivo models; 6) submit the best lead compound to GLP- compliant IND-enabling studies. Concurrent with theabove aims, we will 7) attempt to identify the precise molecular target of the TTPs through affinity studies. At the end of Phase 2, a TTP compound will be ready for IND filing and eventual clinical trials. PUBLIC HEALTH RELEVANCE: Hepatitis B is listed by NIAID as a Small Business High Priority Area of Interest. In response, this is a proposal for the development of a drug that lowers blood serum levels of hepatitis B virus surface antigen. This new drug would function through a different mechanismthan the currently approved anti-hepatitis B drugs, and may be of use in multi-drug cocktails to treat cases of drug-resistant infection, and to extend efficacy. At the end of the proposed project, the novel compound would be ready to enter clinical trials.

Principal Investigator:

Andrea Cuconati
215-489-4918
acuconati.research@hepb.org

Business Contact:

Tong Xiao
215-589-6409
txiao@pharmabridgegroup.com
Small Business Information at Submission:

PHARMABRIDGE, INC.
3805 OLD EASTON ROAD DOYLESTOWN, PA -

EIN/Tax ID: 120470935
DUNS: N/A
Number of Employees: N/A
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No