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SBIR TOPIC 294 PHASE I - DEVELOPMENT OF GLYCOSYLATION-SPECIFIC RESEARCH…

Award Information

Agency:
Department of Health and Human Services
Branch:
N/A
Award ID:
Program Year/Program:
2011 / SBIR
Agency Tracking Number:
N43CO110073
Solicitation Year:
2011
Solicitation Topic Code:
NCI
Solicitation Number:
Small Business Information
DETROIT RANDD, INC.
4683 RAVINE DR BLOOMFIELD HILLS, MI 48301-3640
View profile »
Woman-Owned: Yes
Minority-Owned: Yes
HUBZone-Owned: No
 
Phase 1
Fiscal Year: 2011
Title: SBIR TOPIC 294 PHASE I - DEVELOPMENT OF GLYCOSYLATION-SPECIFIC RESEARCH REAGENTS (ANTIBODIES AND APTAMERS)
Agency: HHS
Contract: N43CO110073
Award Amount: $147,520.00
 

Abstract:

Protein O-GlcNAcylation (N-acetylglucosamine modification), which decreases phosphorylation, plays a critical role in cell-cycle regulation, apoptosis and signal transduction. So far, ~120 O-GlcNAcylated human proteins have been found. To elucidate functions of O-GlcNAcylation, it is necessary to develop site-specific antibodies for O-GlcNAcylated proteins. Many pan-specific antibodies for O-GlcNAcylation are available. However, few site-specific antibodies for O-GlcNAcylated proteins have been successfullyproduced. During the Phase I study, we will synthesize multiple antigen peptides with various features against O-GlcNAcylation sites of c-myc and p53 to test effects of a specific peptide design on the sensitivity and specificity of the antibodies. Sensitivity and specificity of the antibodies will be determined to find out the best peptide design. We will explore utility of the O-GlcNAcylation site-specific antibodies using human breast cell lines, normal (MCF10A) and tumor cells (MCF-7 and N-ras and H-ras MCF10A) with and without treatment with various O-GlcNAcase inhibitors.

Principal Investigator:

Hyesook Kim
248-539-3236
HSKIM@AOL.COM

Business Contact:

Hyesook Kim
248-539-3236
HSKIM@AOL.COM
Small Business Information at Submission:

DETROIT RANDD, INC.
4683 RAVINE DR BLOOMFIELD HILLS, MI 48301-3640

EIN/Tax ID: 383375464
DUNS: N/A
Number of Employees: N/A
Woman-Owned: Yes
Minority-Owned: Yes
HUBZone-Owned: No