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TAS::75 0850::TAS RAPID SCREENING OF INDIVIDUAL CELLS USING SINGLE CELL MASS SPECTROMETRY

Award Information
Agency: Department of Health and Human Services
Branch: National Institutes of Health
Contract: N44CO110098
Agency Tracking Number: N44CO110098
Amount: $1,000,000.00
Phase: Phase II
Program: SBIR
Solicitation Topic Code: NCI
Solicitation Number: N/A
Timeline
Solicitation Year: 2011
Award Year: 2011
Award Start Date (Proposal Award Date): N/A
Award End Date (Contract End Date): N/A
Small Business Information
4878 RONSON CT STE K
SAN DIEGO, CA 92111-1806
United States
DUNS: 159070825
HUBZone Owned: No
Woman Owned: No
Socially and Economically Disadvantaged: No
Principal Investigator
 THOMAS DARLINGTON
 (619) 890-0704
 TOM.DARLINGTON@NANOCOMPOSIX.COM
Business Contact
 THOMAS DARLINGTON
Phone: (619) 890-0704
Email: TOM.DARLINGTON@NANOCOMPOSIX.COM
Research Institution
 Stub
Abstract

A Single Cell time-of-flight Mass Spectrometer (SCMS) that is enabled by the use of a nanoparticle based MALDI matrix will be fabricated and tested. Unlike traditional surface-receptor-based cell phenotyping and identification techniques, the SCMS system will be able to differentiate between cell types that are indistinguishable using traditional antibody based methods. The label-free method will provide molecular information on individual cells that will lead to a new understanding of the molecular variance in heterogeneous cell mixtures. Another mode of operation allows for the use of either fluorescent, nanoparticle or elemental tags (e.g. lanthanides), to provide a highly multi-plexed labeling method using the single cell mass spectrometer as the detector. The SCMS will also have the capability to be coupled to a cytometer which can be used to isolate cell populations of interest prior to mass spectrometry analysis. Here, cell populations labeled with a fluorescent antibody can be further analyzed to measure the variance and identify sub-populations within the tagged cells.

* Information listed above is at the time of submission. *

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