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Development of an in vitro assay as correlate of passive immune protection…

Award Information

Department of Defense
Office for Chemical and Biological Defense
Award ID:
Program Year/Program:
2011 / SBIR
Agency Tracking Number:
Solicitation Year:
Solicitation Topic Code:
Solicitation Number:
Small Business Information
5754 PACIFIC CENTER BLVD, #204 SAN DIEGO, CA 92121-4207
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Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No
Phase 1
Fiscal Year: 2011
Title: Development of an in vitro assay as correlate of passive immune protection against botulinum neurotoxin to minimize use of whole animal testing
Agency / Branch: DOD / CBD
Contract: W81XWH-11-C-0085
Award Amount: $149,949.00


This Small Business Innovation Research Phase I project will develop a non-animal based in-vitro assay for determining the efficacy of Botulinum (BoNT) neutralizing therapeutics; the dual nanopore assay (DNPA). New technologies are currently needed in order to facilitate the development and approval of BoNT vaccines and therapies, and align with the NIH and FDA"s guidance for reducing/replacing the number of vertebrate animals used in medical testing. Currently, the mouse lethality neutralization (MLN) assay is the standard test for determining the efficacy of BoNT neutralizing therapeutics. However, the inherent variability in sensitivity of the MLN assay due to different mouse species, test conditions, and the need to utilize live animal testing severely limit its accuracy and utility. EBS"s DNPA will be capable of monitoring the complete mechanism of all seven BoNT isoforms, making rapid analysis of BoNT neutralizing therapeutics, including complex mixtures, possible.

Principal Investigator:

Eric Ervin
Vice President R&D - EBS Utah
(801) 582-0146

Business Contact:

Tracey Wrightson
Director of Operations
(858) 412-1800
Small Business Information at Submission:

Electronic BioSciences LLC
5754 Pacific Center Blvd Suite 204 San Diego, CA -

EIN/Tax ID: 274484922
Number of Employees:
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No