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Development of a universal method for diagnostic sample inactivation,…

Award Information

Agency:
Department of Defense
Branch:
N/A
Award ID:
Program Year/Program:
2011 / SBIR
Agency Tracking Number:
O2-1059
Solicitation Year:
2009
Solicitation Topic Code:
OSD09-H19
Solicitation Number:
2009.3
Small Business Information
PRESSURE BIOSCIENCES, INC.
14 Norfolk Ave South Easton, MA -
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Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No
 
Phase 2
Fiscal Year: 2011
Title: Development of a universal method for diagnostic sample inactivation, extraction and enrichment of pathogens in arthropod hosts
Agency: DOD
Contract: W81XWH-10-C-0175
Award Amount: $749,988.00
 

Abstract:

We propose to develop an easy-to-use, safe, automated, field-portable high-throughput system, with related methods and consumables, to allow for the simultaneous inactivation and extraction of target molecules from pathogenic organisms, such as viruses, and rickettsia and non-rickettsial bacteria, that propagate and/or disseminate in arthropod hosts (herein referred to as Agents). The proposed System will use a combination of mechanical homogenization, high hydrostatic pressure, elevated temperature, and optimized chemical reagents to inactivate said Agents and extract the target molecules (DNA, RNA and proteins) from host organisms for subsequent separation and detection by a variety of conventional methods. We propose that the System would be equally effective in the field and in a laboratory environment. All sample processing will occur in sealed sample containers. These containers will facilitate sample homogenization, lysis, and rapid extraction of target molecules. Additionally, they will be suitable for sample collection, transportation, and storage (pre- and post analysis). Such containers will help ensure strict chain-of-custody, prevent sample cross-contamination, and reduce the likelihood of exposing personnel to infectious Agents. The System will be made rugged and portable; will be capable of automating the steps of sample inactivation, fractionation, enrichment, and extraction; and will be deployable in the field environment.

Principal Investigator:

Alexander Lazarev
Vice President of R&D
(508) 230-1828
alazarev@pressurebiosciences.com

Business Contact:

Richard Schumacher
President and CEO
(508) 230-1828
rschumacher@pressurebiosciences.com
Small Business Information at Submission:

PRESSURE BIOSCIENCES, INC.
14 Norfolk Ave South Easton, MA -

EIN/Tax ID: 042652826
DUNS: N/A
Number of Employees:
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No