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Handheld Aptamer-Magnetic Bead-Quantum Dot Sensor for Leishmania

Award Information

Department of Defense
Defense Health Program
Award ID:
Program Year/Program:
2011 / SBIR
Agency Tracking Number:
Solicitation Year:
Solicitation Topic Code:
Solicitation Number:
Small Business Information
4100 N.W. LOOP 410 SAN ANTONIO, TX -
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Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No
Phase 2
Fiscal Year: 2011
Title: Handheld Aptamer-Magnetic Bead-Quantum Dot Sensor for Leishmania
Agency / Branch: DOD / DHP
Contract: W81XWH-10-C-0179
Award Amount: $709,977.00


Operational Technologies Corporation (OpTech) has completed a successful Phase I SBIR contract in which 72 new candidate DNA aptamer sequences against Leishmania major were developed and screened by ELISA-like plate assay. Several pilot assays were developed from the highest affinity candidate aptamers using a commercially available handheld and battery-operated fluorometer. In Phase II, OpTech plans to fully develop and deliver to the DoD two or more rapid (15 minute) ultrasensitive one-step plastic-adherent DNA aptamer-magnetic bead (MB) fluorescent sandwich assays (Bruno et al., J. Fluorescence 19:427-435, 2009) for L. major and L. infantum and a visceral leishmaniasis (39 kD kinesin) aptamer assay. In Phase I, this assay format demonstrated detection of Campylobacter and Listeria to<10 cfu/mL and between 10-100 Leishmania promastigotes. OpTech will optimize its lyophilized assay performance with respect to specificity, sensitivity and shelf life of>2 years at 35oC by systematic study of DNA aptamer, magnetic bead, and fluorescent nanoparticle reagent concentrations. OpTech will also evaluate trehalose or other excipient level effects along with innovative vacuum or air-tight packaging of the lyophilized assays. Finally, OpTech will test the assays against various species of live Leishmania and compare to gold standard ELISA and PCR assays.

Principal Investigator:

John G. Bruno
Senior Scientist
(210) 731-0015

Business Contact:

William M. Henderson
Chief Financial Officer
(210) 731-0015
Small Business Information at Submission:

Operational Technologies Corporation
4100 N. W. Loop 410 Suite 230 San Antonio, TX 78229-4253

EIN/Tax ID: 742406648
Number of Employees:
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No