A Rapid Coliform Detector
ORBITEC, in collaboration with Lucigen, proposes a rapid genetic detector for spaceflight water systems to enable real-time detection of E. coli with minimal consumables and crew time. The Rapid Coliform Detector (RCD) amplifies the genetic material in a liquid sample to allow near real-time identification of specific genetic sequences, in this case, that of E. coli. This easy-to-use device incorporates a patented polymerase enzyme that enables rapid RNA amplification by reagents with superior long-term shelf life and thermal stability. A color change indicator will show the presence or absence of coliform bacteria in the water within 30 minutes. The results of the Phase 1 will be test data from prototype test kits and chemical reagents for rapid coliform detection which brings the RCD to TRL 4. The anticipated results of the Phase 2 are a flight-like prototype of the complete test kit and reaction chamber, performance test results at 1g, and reduced gravity operational test results, which bring the technology to TRL 6.
Small Business Information at Submission:
Orbital Technologies Corporation
Space Center, 1212 Fourier Drive Madison, WI -
Number of Employees: