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A Rapid Coliform Detector

Award Information

National Aeronautics and Space Administration
Award ID:
Program Year/Program:
2012 / SBIR
Agency Tracking Number:
Solicitation Year:
Solicitation Topic Code:
Solicitation Number:
Small Business Information
Orbital Technologies Corporation (ORBITE
Space Center, 1212 Fourier Drive Madison, WI 53717-
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Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No
Phase 1
Fiscal Year: 2012
Title: A Rapid Coliform Detector
Agency: NASA
Contract: NNX12CE71P
Award Amount: $125,000.00


ORBITEC, in collaboration with Lucigen, proposes a rapid genetic detector for spaceflight water systems to enable real-time detection of E. coli with minimal consumables and crew time. The Rapid Coliform Detector (RCD) amplifies the genetic material in a liquid sample to allow near real-time identification of specific genetic sequences, in this case, that of E. coli. This easy-to-use device incorporates a patented polymerase enzyme that enables rapid RNA amplification by reagents with superior long-term shelf life and thermal stability. A color change indicator will show the presence or absence of coliform bacteria in the water within 30 minutes. The results of the Phase 1 will be test data from prototype test kits and chemical reagents for rapid coliform detection which brings the RCD to TRL 4. The anticipated results of the Phase 2 are a flight-like prototype of the complete test kit and reaction chamber, performance test results at 1g, and reduced gravity operational test results, which bring the technology to TRL 6.

Principal Investigator:

Ross W. Remiker
Principal Investigator

Business Contact:

Thomas Crabb
Business Official
Small Business Information at Submission:

Orbital Technologies Corporation
Space Center, 1212 Fourier Drive Madison, WI -

EIN/Tax ID: 391628664
Number of Employees:
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No