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Engineering Photosynthetic Cyanobacteria to Produce the Energy-Dense Fuel…

Award Information

Agency:
Department of Agriculture
Branch:
N/A
Award ID:
Program Year/Program:
2012 / SBIR
Agency Tracking Number:
2012-00437
Solicitation Year:
2012
Solicitation Topic Code:
8.8
Solicitation Number:
USDA-NIFA-SBIR-003497
Small Business Information
DAST, LLC
815 Medary Avenue, Suite 201 Brookings, SD 57006-1303
View profile »
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No
 
Phase 1
Fiscal Year: 2012
Title: Engineering Photosynthetic Cyanobacteria to Produce the Energy-Dense Fuel Farnesene from Carbon Dioxide
Agency: USDA
Contract: 2012-00437
Award Amount: $99,875.00
 

Abstract:

The goal of the project is to genetically engineer photosynthetic cyanobacteria that will synthesize farnesene from carbon dioxide. The work will then involve shutting down pathways that compete for carbon in an attempt to maximize the amount of farnesene that can be produced. Then we will do tolerance testing by culturing the cyanobacteria in varying concentrations of the end product(s) in order to determine the optimal culturing conditions. This work will develop an engineered cyanobacterium capable of converting CO2 into farnesene (C15H24), a long-chain hydrocarbon with an energy density of 47 MJ/Kg, heat of vaporization of 0.24 MJ/Kg, and octane of 89.7. The cells would excrete farnesene into the recirculating culture fluid, where it would be recovered by low-cost phase separation for use as an energy-dense, infrastructure compatible biofuel. We have already established the validity of this "cyanofactory" platform by engineering Anabaena to produce the ten-carbon alcohol linalool. Farnesene has a much higher energy density and thus would be a more desirable 3rd generation biofuel.

Principal Investigator:

Tyler Remund
605-695-6631
tyler@southdakotainnovation.com

Business Contact:

Mark Luecke
President
605-366-3151
mark@southdakotainnovation.com
Small Business Information at Submission:

DAST
815 MEDARY AVE STE 201 Brookings, SD 57006-1303

EIN/Tax ID: 270249495
DUNS: N/A
Number of Employees:
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No