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Yeast-Based High-Throughput Screens for Inhibitors of Key Flaviviral Enzymes

Award Information

Department of Defense
Award ID:
Program Year/Program:
2012 / SBIR
Agency Tracking Number:
Solicitation Year:
Solicitation Topic Code:
Solicitation Number:
Small Business Information
Agave BioSystems, Inc.
401 E. State Street Suite 200 Ithaca, AK 14850-
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Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No
Phase 1
Fiscal Year: 2012
Title: Yeast-Based High-Throughput Screens for Inhibitors of Key Flaviviral Enzymes
Agency: DOD
Contract: W81XWH-12-C-0040
Award Amount: $100,000.00


With no clinically approved antiviral therapy available for treatment of flavivirus infections, the development of efficient vaccines and antiviral agents for prevention and treatment of infections is an urgent health priority. A combination of structure-based in silico models, in vitro and cell-based assays has led in the last decade to the identification of promising new compounds with antiviral properties, for the most part targeting one of the key enzymatic functions essential to the life cycle of these viruses. In this context development of highly-performing assays with increased screening capacity is seen as a valuable tool needed to extend screening campaigns. Agave BioSystems proposes to develop yeast-based assays for the high-throughput screening of chemical inhibitors of the flaviviral NS2B/NS3 protease activity and both the RNA methyltransferase activity and RNA polymerase activity of NS5. Key advantages of yeast-based assays are the direct screening in a cellular environment, strong statistical performance in microplate formats with no reagent aging, and most importantly extremely simple and cost-effective scalability for high-throughput campaigns.

Principal Investigator:

Julien Fey
Senior Scientist
(607) 272-0002

Business Contact:

Noe Salazar
(512) 656-6200
Small Business Information at Submission:

Agave BioSystems, Inc.
P.O. Box 100 Ithaca, NY -

EIN/Tax ID: 161551819
Number of Employees:
Woman-Owned: No
Minority-Owned: Yes
HUBZone-Owned: Yes