USA flag logo/image

An Official Website of the United States Government

Microscale Tip Device for Rapid Concentration and Storage of PCR-ready DNA from…

Award Information

Agency:
Department of Health and Human Services
Branch:
N/A
Award ID:
Program Year/Program:
2012 / SBIR
Agency Tracking Number:
R43GM099347
Solicitation Year:
2012
Solicitation Topic Code:
NIGMS
Solicitation Number:
PA11-096
Small Business Information
NanoFacture, Inc.
16301 NE 8th St Ste 110 Bellevue, WA 98008
View profile »
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No
 
Phase 1
Fiscal Year: 2012
Title: Microscale Tip Device for Rapid Concentration and Storage of PCR-ready DNA from B
Agency: HHS
Contract: 1R43GM099347-01A1
Award Amount: $150,000.00
 

Abstract:

DESCRIPTION (provided by applicant): The current SBIR Phase I proposal addresses a need for the rapid and simple sample preparation of genomic DNA from human saliva and blood through a novel technology: microscale tip enrichment probes that employ electric fields and capillary action. With the action of a microtip-installed device, DNA contained in a given saliva sample can be selectively captured without centrifugation on the proposed microtip, and stored as a dried form for long- term preservation and delivery. The preserved DNA on microtips is released into a desired medium for PCR analysis without additional purification steps. Therefore, this innovative technology enables the one-step concentration of DNA from human saliva and blood as a purified, durable form. This application describes a feasibility study of the proposed method and system to explore and optimize the protocol of centrifuge-free preparation of PCR-ready DNA. PUBLIC HEALTH RELEVANCE: This proposal aims to conduct a feasibilitystudy for a portable device capable of concentrating and purifying human genomic DNA from saliva without centrifuge. The success of the project would provide a convincing rationale and form a technical basis for the proposed methodology for rapid and simple preparation of PCR-ready DNA from various sample specimens with a high throughput at a low cost.

Principal Investigator:

Kyong-hoon Lee
425-269-6051
hoonlee@nano-facture.com

Business Contact:

Jiyoung Kang
425-830-0530
j.kang@nano-facture.com
Small Business Information at Submission:

NANOFACTURE, INC.
16301 NE 8th St Ste 110 Bellevue, WA 98008

EIN/Tax ID: 120441683
DUNS: N/A
Number of Employees: N/A
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No