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Highly Fluorescent Magnetic Nanoclusters for Effective and Multiplexed…

Award Information

Agency:
Department of Health and Human Services
Branch:
N/A
Award ID:
Program Year/Program:
2012 / SBIR
Agency Tracking Number:
R43GM100602
Solicitation Year:
2012
Solicitation Topic Code:
NIGMS
Solicitation Number:
PA10-150
Small Business Information
NVIGEN, INC.
942 RICH PL MOUNTAIN VIEW, CA 94040-2477
View profile »
Woman-Owned: Yes
Minority-Owned: No
HUBZone-Owned: No
 
Phase 1
Fiscal Year: 2012
Title: Highly Fluorescent Magnetic Nanoclusters for Effective and Multiplexed Separation
Agency: HHS
Contract: 1R43GM100602-01A1
Award Amount: $201,157.00
 

Abstract:

DESCRIPTION (provided by applicant): The ability to effectively isolate rare cells and identify subset of cell characteristics could provide significant prognostic and therapeutic implications ranging from cancer diagnostics to stem cell therapies. The rare nature of these cells imposes particular challenge on the functionality and robustness of the separation and analysis methodology. Nanotechnology could provide novel and practical solutions to meet such challenge. The broad objective of this proposal isto develop superior nanoparticle reagents in combination with a unique separation scheme that could be applied for highly effective and multiplexed separation of rare cells. These nanoparticle agents will be based on the highly fluorescent magnetic nanoclusters (HFMN) with superior fluorescent properties and precisely tunable magnetic moments. HFMN will provide an excellent platform technology that not only allows optimized magnetic control for effective separation of rare cells, but also facilitates multiplexed cell separation using a specifically designed external magnetic field gradient control. In addition, the superior fluorescent signal of HFMN will allow simultaneous tracking and in situ biomedical analysis for cell type identification and for diagnostic or therapeutic evaluations. The specific aims are: 1) Synthesize HFMN with optimal fluorescent brightness and tunable magnetic properties. 2) Apply HFMN for efficient rare cell separation and identification in whole blood samples. 3) Demonstrate theadvanced capability of HFMN in combination with a specifically designed external magnetic field gradient control for multiplexed separation and identification of rare cells in whole blood samples. Such high quality multifunctional nanoparticles and the unique magnetic separation scheme when made commercially available, could significantly advance the current technology in rare cell separation and analysis, and ultimately contribute to improved cellular diagnosis and therapy for many patients. PUBLIC HEALTH RELEVANCE: The goal of this research is to develop novel nanoparticle reagents, the highly fluorescent magnetic nanoclusters, and a specifically designed magnetic separation scheme for highly effective and multiplexed separation and identification of rare cells. Success in achieving this goal could significantly advance the current technology in rare cell separation and analysis including circulating tumor cells and therapeutic cells such as T-cells and stem cells, and ultimately contribute to improved cellular diagnosis and therapy for many patients.

Principal Investigator:

Aihua Fu
408-505-9232
aihua.fu@gmail.com

Business Contact:

Aihua Fu
408-505-9232
aihua.fu@gmail.com
Small Business Information at Submission:

NVIGEN, INC.
942 RICH PL MOUNTAIN VIEW, CA 94040-2477

EIN/Tax ID: 127480426
DUNS: N/A
Number of Employees: N/A
Woman-Owned: Yes
Minority-Owned: No
HUBZone-Owned: No