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Affinity Matrix System for Large-Scale Protein Purification

Award Information

Agency:
Department of Defense
Branch:
N/A
Award ID:
Program Year/Program:
2013 / SBIR
Agency Tracking Number:
C122-102-0080
Solicitation Year:
2012
Solicitation Topic Code:
CBD12-102
Solicitation Number:
2012.2
Small Business Information
Luna Innovations Incorporated
1 Riverside Circle Suite 400 Roanoke, VA 24016-
View profile »
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No
 
Phase 1
Fiscal Year: 2013
Title: Affinity Matrix System for Large-Scale Protein Purification
Agency: DOD
Contract: W911NF-13-C-0032
Award Amount: $100,000.00
 

Abstract:

Though vast improvements have been made in various protein expression platforms, limitations arise in the implementation of current purification methods. Traditional chromatographic methods are time intensive, carry heavy material costs, and are difficult to scale - reducing the benefits achieved in protein expression. Affinity-binding methods are promising for scale-up and swiftness of purification, but the necessary resins can be expensive and difficult to adapt to purify proteins from new expression platforms. To respond to this need, Luna proposes the development of a low-cost affinity matrix with a high surface area to provide substantial binding regions for targeted proteins of interest. This system will allow for the purification of any protein expressed with the cellulose binding module and intein self-cleaving tag, an expression possible with many proteins in a wide variety of host organisms. The implementation of this low-cost affinity matrix and widely applicable purification system will allow for fast, simple, inexpensive purification of any target protein.

Principal Investigator:

Michael Danilich
Principal Investigator
(434) 220-0159
danilichm@lunainnovations.com

Business Contact:

Maggie Hudson
Senior Contracts Administrator
(434) 483-4254
submissions305@lunainnovations.com
Small Business Information at Submission:

Luna Innovations Incorporated
1 Riverside Circle Suite 400 Roanoke, VA -

EIN/Tax ID: 541560050
DUNS: N/A
Number of Employees:
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No