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T7 RNA polymerase engineering and RNA amplification

Award Information

Agency:
Department of Health and Human Services
Branch:
N/A
Award ID:
71677
Program Year/Program:
2007 / STTR
Agency Tracking Number:
GM072412
Solicitation Year:
N/A
Solicitation Topic Code:
N/A
Solicitation Number:
N/A
Small Business Information
Accacia International LLC
One Tech Plaza 2113 Wells Branch Parkway, Suite 6900 Austin, TX -
View profile »
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No
 
Phase 2
Fiscal Year: 2007
Title: T7 RNA polymerase engineering and RNA amplification
Agency: HHS
Contract: 2R42GM072412-02
Award Amount: $1,348,691.00
 

Abstract:

DESCRIPTION (provided by applicant): Expanding use of microarray systems for global gene expression profiling is leading biological research, providing diagnostic and prognostic value to physicians and facilitating target discovery during drug and vaccine development. There is strong interest in extending this microarray capability to more RNA-limited material to the point that a single cell can be profiled with confidence. The most widely used method for preparing samples for microarray analysis, which use s cDNA synthesis from mRNA followed by T7 RNA polymerase-based amplification, currently lacks the necessary sensitivity to meet these emerging needs. To overcome these methodological limitations, we propose to increase the final amplified RNA yield by 1) i mproving the efficiency of T7 RNA polymerase using structure-based engineering with directed evolution of function, 2) by optimizing the design of the promoter-template construct for specific amplification needs and 3) by re-standardization of cDNA synthes is for lower RNA inputs. The combined improvements are expected to lower the cell-limited RNA sample requirement by 100-fold compared to current, commercially available kits. This will enable microarray gene analysis with as little as 1 ng of total RNA wit h a single round of amplification or microarray profiling from a single cell (-10 pg of total RNA) after two rounds of amplification.

Principal Investigator:

Andrew D. Ellington
5122323426
ANDY.ELLINGTON@MAIL.UTEXAS.EDU

Business Contact:

Eula Dequeira
Small Business Information at Submission:

ACCACIA INTERNATIONAL, INC.
ACCACIA INTERNATIONAL, INC. 2113 WELLS BRANCH PKWY, STE 6900 AUSTIN, TX 78728

EIN/Tax ID: 742515119
DUNS: N/A
Number of Employees: N/A
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No
Research Institution Information:
UNIVERSITY OF TEXAS AT AUSTIN
UNIVERSITY OF TEXAS AUSTIN
PO BOX 7726
AUSTIN, TX 78713 1980
RI Type: Nonprofit college or university