Improving Glycosyltransferase by RACHITT Gene Shuffling
DESCRIPTION (provided by applicant): Glycosyltransferases involved in linking
polyketide macrolactones with deoxysugars are key enzymes for combinatorial
biosynthesis of novel macrolide anti-infective agents. In this proposal, a new
gene shuffling technology, RACHITT, is applied to the desosarninyl transferase
(Des VII) from the pikromycin biosynthetic pathway of Streptomyces venezuelae.
Des VII is an enzyme with remarkable inherent substrate flexibility, and we
expect to expand further its substrate spectrum to include 16-membered ring
macrolactones. This technology is expected to provide glycosyltransferase
enzymes that can catalyze the biosynthesis of 12-, 14-, and 16-membered ring
macrolides. Moreover, we expect to generate a recombinant DesVII that has a
higher enzymatic activity toward the 14-membered ring macrolactone narbonolide.
This will be a key step in combinatorial biosynthesis of ketolide antibiotics
that have a potential to combat multi-drug resistant bacterial pathogens.
PROPOSED COMMERCIAL APPLICATION: NOT AVAILABLE
Small Business Information at Submission:
Principal Investigator:Yongquan Xue
ACERA BIOSCIENCES, INC.
603 E LAKE ST, STE 200F WAYZATA, MN 55391
Number of Employees: