USA flag logo/image

An Official Website of the United States Government

Improving Glycosyltransferase by RACHITT Gene Shuffling

Award Information

Agency:
Department of Health and Human Services
Branch:
N/A
Award ID:
54574
Program Year/Program:
2001 / SBIR
Agency Tracking Number:
1R43GM064312-01
Solicitation Year:
N/A
Solicitation Topic Code:
N/A
Solicitation Number:
N/A
Small Business Information
ACERA BIOSCIENCES, INC.
ACERA BIOSCIENCES, INC. 603 E LAKE ST, STE 200F WAYZATA, MN 55391
View profile »
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No
 
Phase 1
Fiscal Year: 2001
Title: Improving Glycosyltransferase by RACHITT Gene Shuffling
Agency: HHS
Contract: N/A
Award Amount: $100,000.00
 

Abstract:

DESCRIPTION (provided by applicant): Glycosyltransferases involved in linking polyketide macrolactones with deoxysugars are key enzymes for combinatorial biosynthesis of novel macrolide anti-infective agents. In this proposal, a new gene shuffling technology, RACHITT, is applied to the desosarninyl transferase (Des VII) from the pikromycin biosynthetic pathway of Streptomyces venezuelae. Des VII is an enzyme with remarkable inherent substrate flexibility, and we expect to expand further its substrate spectrum to include 16-membered ring macrolactones. This technology is expected to provide glycosyltransferase enzymes that can catalyze the biosynthesis of 12-, 14-, and 16-membered ring macrolides. Moreover, we expect to generate a recombinant DesVII that has a higher enzymatic activity toward the 14-membered ring macrolactone narbonolide. This will be a key step in combinatorial biosynthesis of ketolide antibiotics that have a potential to combat multi-drug resistant bacterial pathogens. PROPOSED COMMERCIAL APPLICATION: NOT AVAILABLE

Principal Investigator:

Yongquan Xue

Business Contact:


9524754929
DTAUS@ACERBIO.COM
Small Business Information at Submission:

ACERA BIOSCIENCES, INC.
603 E LAKE ST, STE 200F WAYZATA, MN 55391

EIN/Tax ID: 411944324
DUNS: N/A
Number of Employees: N/A
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No