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MULTIWELL COLORIMETRIC ASSAY FOR DNA REPAIR PROTEIN.

Award Information

Agency:
Department of Health and Human Services
Branch:
N/A
Award ID:
60079
Program Year/Program:
2002 / SBIR
Agency Tracking Number:
CA096392
Solicitation Year:
N/A
Solicitation Topic Code:
N/A
Solicitation Number:
N/A
Small Business Information
Active Motif, Inc.
1914 Palomar Oaks Way Suite 150 Carlsbad, CA 92008-6509
View profile »
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No
 
Phase 1
Fiscal Year: 2002
Title: MULTIWELL COLORIMETRIC ASSAY FOR DNA REPAIR PROTEIN.
Agency: HHS
Contract: 1R43CA096392-01A1
Award Amount: $99,380.00
 

Abstract:

DESCRIPTION (provided by applicant): DNA damages are key events during carcinogenesis. Deficiencies in repairing damaged DNA represent a poor prognosis during tumorigenesis and/or cancer treatment. Besides monitoring DNA damage formation, it would be helpful to monitor DNA repair protein activity. We propose to develop a new technology that allows for high-throughput and fast analysis of the most common DNA repair proteins. We will use a damaged DNA probe immobilized onto a 96-well microplate to capture DNA repair proteins in an extract from cells or tissues. Six biotinylated oligonucleotides containing different DNA damage types will be synthesized. The DNA-protein binding conditions will be developed by using purified recombinant DNA repair proteins. Antibodies raised specifically against DNA repair proteins will identify the bound protein and yield a quantitative result in two hours. Finally, the procedure will be applied to relevant samples such as extracts from damaging agent-treated cell lines and disease models. Such assay will help elucidate the molecular mechanisms of the DNA damage and repair pathways. It would also provide scientists and clinicians with a unique tool to answer seminal questions in risk assessment in a fraction of the time normally required.

Principal Investigator:

Jacques T. Weissman

Business Contact:

Kent Johnson
7604311263
JOHNSON@ACTIVEMOTIF.COM
Small Business Information at Submission:

ACTIVE MOTIF, LLC
ACTIVE MOTIF, LLC 5431-C AVENIDA ENCINAS CARLSBAD, CA 92008

EIN/Tax ID: 330858864
DUNS: N/A
Number of Employees: N/A
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No