You are here

Recombinant antibodies for antibody arrays

Award Information
Agency: Department of Health and Human Services
Branch: National Institutes of Health
Contract: 1R43GM110868-01
Agency Tracking Number: R43GM110868
Amount: $297,840.00
Phase: Phase I
Program: SBIR
Solicitation Topic Code: NIGMS
Solicitation Number: PA11-215
Timeline
Solicitation Year: 2014
Award Year: 2014
Award Start Date (Proposal Award Date): N/A
Award End Date (Contract End Date): N/A
Small Business Information
1601 Research Boulevard
ROCKVILLE, MD 20850-3126
United States
DUNS: 113033224
HUBZone Owned: No
Woman Owned: No
Socially and Economically Disadvantaged: No
Principal Investigator
 JOHN KENTEN
 (240) 631-2522
 jkenten@mesoscale.com
Business Contact
 JOHN KENTEN
Phone: (240) 631-2522
Email: jkenten@mesoscale.com
Research Institution
 Stub
Abstract

DESCRIPTION (provided by applicant): We propose to develop a rapid, low cost and high-throughput method for generating recombinant engineered antibodies with improved properties for proteomic and immunoassay applications. Engineered antibody constructs such as recombinant Fab fragments and chimeric antibodies will be generated and expressed by directly modifying the antibody genes in antibody-producing hybridoma cell lines, thereby eliminating the need for gene cloning and transfection. In this Phase I SBIR, we will demonstrate feasibility by modifying ten (10) hybridoma cell lines that produce antibodies used in MSD's commercial immunoassay kits. We will demonstrate that we can modify the cell lines so that they express their antibodies as recombinant Fabfragments or recombinant chimeras comprising Fab fragments linked to protein domains that enable site- specific labeling. The use of antibodies from established kits will make it straightforward to directly compare the assay performance of the new

* Information listed above is at the time of submission. *

US Flag An Official Website of the United States Government