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Functional-selection of affinity reagents against DNA-protein complexes using targeted chromatin sequences

Award Information
Agency: Department of Health and Human Services
Branch: National Institutes of Health
Contract: 1R41DK104602-01
Agency Tracking Number: R41DK104602
Amount: $218,694.00
Phase: Phase I
Program: STTR
Solicitation Topic Code: NIDDK
Solicitation Number: PA14-072
Timeline
Solicitation Year: 2014
Award Year: 2014
Award Start Date (Proposal Award Date): N/A
Award End Date (Contract End Date): N/A
Small Business Information
688 E MAIN ST, STE 2A
BRANFORD, CT 06405-2971
United States
DUNS: 78402317
HUBZone Owned: No
Woman Owned: No
Socially and Economically Disadvantaged: No
Principal Investigator
 MICHAEL WEINER
 (203) 458-2844
 mweiner@axiomxinc.com
Business Contact
 MICHAEL WEINER
Phone: (203) 606-5394
Email: mweiner@axiomxinc.com
Research Institution
 UNIVERSITY OF ILLINOIS AT CHICAGO
 
UNIVERSITY OF ILLINOIS AT CHICAGO 310 AOB, M/C 672
CHICAGO, IL 60612-7224
United States

 () -
 Nonprofit College or University
Abstract

DESCRIPTION (provided by applicant): Site-specific binding of proteins to DNA plays an important role in cell development, cell signaling, the cell cycle, and diseases such as cancer. There are two overarching goals to this proposal: (1) to develop a meansfor the identification of proteins bound to specific DNA sequences, and (2) to develop a better method for the identification of immunoprecipitation (IP) functional affinity reagents against DNA-bound proteins found on a complex antigen source. We proposeto apply the proof-of-principle using the interaction of transcription factors (TFs) and their specific DNA binding sites as a model. TFs are one major example of such DNA-binding proteins. Once bound to their binding site(s), TF proteins can regulate thetranscription of genes, thereby making individual genes either more or less active. Although this is a proposal to develop a better means toward obtaining functional affinity reagents against proteins when they are bound to DNA, the method itself can

* Information listed above is at the time of submission. *

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