Label-free platform for applications in drug discovery

DESCRIPTION: Incremental improvements in label free screening technologies over the past decade have not produced the capability necessary to support the changing needs of drug discovery programs. Persistent artifacts related to non-specific binding, promiscuous binding, precipitation, complex binding, tubing retention, aggregation, and sub-optimally designed platforms continue to hinder progress. Substantial progress has been made in establishing methodologies that maximize the value of the data that is currently available. The proposed platform encompasses a variety of novel adaptations including a dual mode detector exploiting surface plasmon resonance (SPR), which is sensitive to changes at the surface, and bulk solution-sensitive critical angle refractometry (CAR). Taylor dispersion-based continuous titrations (OneStepTM) and stepped analyte titrations (FastStepTM) are combined with an innovative thermal gradient capability to provide a high throughput thermodynamic screening format (DH, DS, DCp) ca