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Development of novel methods and reagents for surveillence of prion infection

Award Information

Agency:
Department of Defense
Branch:
Office of the Secretary of Defense
Award ID:
76591
Program Year/Program:
2005 / SBIR
Agency Tracking Number:
O052-H05-3097
Solicitation Year:
N/A
Solicitation Topic Code:
N/A
Solicitation Number:
N/A
Small Business Information
ADLYFE, INC.
9430 Key West Avenue, Suite 210 Rockville, MD 20850
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Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No
 
Phase 1
Fiscal Year: 2005
Title: Development of novel methods and reagents for surveillence of prion infection
Agency / Branch: DOD / OSD
Contract: W81XWH-06-C-0065
Award Amount: $100,000.00
 

Abstract:

Adlyfe Inc. has developed a highly sensitive fluorescence based test, called the Misfolded Protein Diagnostic (MPD) Assay, for the misfolded prion protein associated with infectivity. The femtomolar sensitivity of the test enables the presymptomatic detection in blood, which can be performed ante mortem in routine surveillance of live animals and in blood screening of the human blood supply. The MPD Assay does not rely on antibodies but mimics the folding activity of prions utilizing a target binding peptide. The amplification of the signal through nucleation of folding of other added binding peptides, allows for the extreme sensitivity of the detection method to be realized. This has been demonstrated in controlled in vitro biophysical studies, controlled models of disease in hamsters and mice, and in endemic disease with blood samples from scrapie sheep, BSE-infected cows and monkey and human sCJD. Current protocols and methodology have been geared toward laboratory bench execution of the MPD assay. Here, we propose to move this exciting new technology forward to a more robust integrated assay system that will ultimately encompass software for data analysis and reporting.

Principal Investigator:

Cindy S. Orser
VP Research & Development
3014248344
corser@adlyfe.com

Business Contact:

Richard Cliff
COO
3014248344
rcliff@adlyfe.com
Small Business Information at Submission:

ADLYFE, INC.
9430 Key West Avenue, Suite 210 Rockville, MD 20850

EIN/Tax ID: 200204901
DUNS: N/A
Number of Employees:
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No