Novel one-step purification with a sortase fusion
DESCRIPTION (provided by applicant): A new protein fusion expression system was conceptualized and is being developed to provide tag-free recombinant protein purification via one-step chromatography. Phase I of this project includes optimization of the activity of the key component in the fusion construct, streamlining the target cloning method, and applications in bacteria expression system. Because this purification method is simple, low cost, time saving, Phase II of this project will advance the one-step method for eukaryotic expression and apply the method to producing proteins for proteomic studies and biotransformation applications.
Small Business Information at Submission:
Advanced Protein Labs
Advanced Protein Labs 505 Coast Blvd S, Ste 412 La Jolla, CA 92037
Number of Employees: