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Combinatorial Molecular Array Analysis via MEF/MALDI/MS

Award Information

Agency:
Department of Health and Human Services
Branch:
N/A
Award ID:
70735
Program Year/Program:
2004 / SBIR
Agency Tracking Number:
CA110680
Solicitation Year:
N/A
Solicitation Topic Code:
N/A
Solicitation Number:
N/A
Small Business Information
Agave BioSystems, Inc.
P.O. Box 100 Ithaca, NY 14850-
View profile »
Woman-Owned: No
Minority-Owned: Yes
HUBZone-Owned: Yes
 
Phase 1
Fiscal Year: 2004
Title: Combinatorial Molecular Array Analysis via MEF/MALDI/MS
Agency: HHS
Contract: 1R43CA110680-01
Award Amount: $99,510.00
 

Abstract:

DESCRIPTION (provided by applicant): Despite numerous advances in high throughput screening (HTS) and system automation, the identification of specific ligands remains time consuming and resource intensive. A major obstacle to more efficient screening of potential ligands is the current requirement for each target protein to be screened individually. A highly effective method for identifying proteins bound to solid supports is matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS). But variability in protein-matrix interactions can make accurate assessments of protein binding difficult, since the absence of mass signal could signify either the absence of binding or the poor desorption/ionization behavior of a particular protein/matrix pair. This ambiguity can be resolved, however, by employing a second, independent measure of binding that is not subject to the same protein-to-protein variability. To this end, we propose the development of a novel discovery platform for the combinatorial analysis of molecular arrays, incorporating metal enhanced fluorescence (MEF) to detect protein binding, and MALDI mass spectrometry to identify the proteins involved.

Principal Investigator:

Louis Hom
6072720002
LHOM@AGAVEBIO.COM

Business Contact:


5126711369
Small Business Information at Submission:

AGAVE BIOSYSTEMS
AGAVE BIOSYSTEMS BOX 80010 AUSTIN, TX 78708

EIN/Tax ID: 161551819
DUNS: N/A
Number of Employees: N/A
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No