Immunological and dna hybridization assays which employ specific antibody and dna probes are proving to be very valuable in the identification of pathogens and in the diagnosis of genetic diseases. the objective of this project is to improve the sensitivity, safety, and convenience of diagnostic… More

Major objectives of this research are to (1) isolate 100 mg of homogeneous dihydrofolate reductase (dhfr) from candida albicans, (2) crystallize the enzyme, and (3) determine its three-dimensional structure using x-ray diffraction methods.armed with the resulting detailed knowledge of the… More

Major objectives of this research are to (1) isolate 100 mg of homogeneous dihydrofolate reductase (dhfr) from pseudomonas aeruginosa, (2) crystallize the enzyme, and (3) determine its three-dimensional structure using x-ray diffraction methods. armed with the resulting detailed knowledge of the… More

This project describes the isolation of dna probes linked tothe site in the human x chromosome involved in fragility of the chromosome under some growth conditions ("fragile x site") and known to be associated with a high incidence of mental retardation. such sequences may be useful in under-… More

A characteristic feature of many rheumatic diseases is the presence of circulating antinuclear antibodies. assays based on the measurement of these antibodies are established in the clinical laboratory for the diagnosis of these diseases, but often without the benefit of having well-characterized or… More

The ultimate objective of this project is to utilize the information available in the three-dimensional crystal structure of an enzyme in the rational design of drugs whichwill be effective against plasmodium falciparum. the specific enzyme target is the bifunctional dihydrofolate… More