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MAJOR OBJECTIVES OF THIS RESEARCH ARE TO (1) ISOLATE 100 MG OF HOMOGENEOUS…

Award Information

Agency:
Department of Health and Human Services
Branch:
N/A
Award ID:
4683
Program Year/Program:
1986 / SBIR
Agency Tracking Number:
4683
Solicitation Year:
N/A
Solicitation Topic Code:
N/A
Solicitation Number:
N/A
Small Business Information
Agouron Pharmaceuticals Inc.
505 Coast Blvd La Jolla, CA 92037
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Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No
 
Phase 1
Fiscal Year: 1986
Title: MAJOR OBJECTIVES OF THIS RESEARCH ARE TO (1) ISOLATE 100 MG OF HOMOGENEOUS DIHYDROFOLATE REDUCTASE (DHFR) FROM PSEUDOMONAS AERUGINOSA, (2) CRYSTALLIZE THE ENZYME, AND (3) DETERMINE ITS THREE-DIMENSIONAL STRUCTURE USING X-RAY DIFFRACTION METHODS.
Agency: HHS
Contract: N/A
Award Amount: $50,000.00
 

Abstract:

MAJOR OBJECTIVES OF THIS RESEARCH ARE TO (1) ISOLATE 100 MG OF HOMOGENEOUS DIHYDROFOLATE REDUCTASE (DHFR) FROM PSEUDOMONAS AERUGINOSA, (2) CRYSTALLIZE THE ENZYME, AND (3) DETERMINE ITS THREE-DIMENSIONAL STRUCTURE USING X-RAY DIFFRACTION METHODS. ARMED WITH THE RESULTING DETAILED KNOWLEDGE OF THE GEOMETRICAL AND TOPOGRAPHICAL PROPERTIES OFPSEUDOMONAS DHFR IN RELATION TO THE KNOWN STRUCTURE OF THE CORRESPONDING VERTEBRATE ENZYME, AGOURON TECHNOLOGY WILL DESIGN SPECIFIC, PHARMACOLOGICALLY ACTIVE INHIBITORS THAT SELECTIVELY CRIPPLE THE BACTERIAL REDUCTASE WITHOUT INTERFERING WITH ONE-CARBON METABOLISM IN THE HOST. SUCH NOVEL INHIBITORS WILL ADDRESS THE CURRENT CRITICAL NEED FOR NEW ANTIMICROBICS WITH ENHANCED ACTIVITY AGAINST PSEUDOMONASAERUGINOSA. INTERMEDIATE OBJECTIVES OF PHASE I RESEARCH ARETO: (1) ISOLATE P. AERUGINOSA DHFR OF SUFFICIENT PURITY ANDIN SUFFICIENT QUANTITY FOR BIOCHEMICAL CHARACTERIZATION AND FOR PROTEIN SEQUENCE DETERMINATION; (2) CHARACTERIZE THE INTERACTION OF PURIFIED P. AERUGINOSA DHFR WITH THE ANTIFOLATE TRIMETHOPRIM (TMP) IN TERMS OF ABSOLUTE AFFINITY AND KI FOR THE DHF THF CONVERSION; AND (3) CLONE THE STRUCTURAL GENE FOR P. AERUGINOSA DHFR.

Principal Investigator:

David a matthews
PRINCIPAL INVESTIGATOR
6194561623

Business Contact:

Small Business Information at Submission:

Agouron Technology Corp
505 Coast Blvd South La Jolla, CA 92037

EIN/Tax ID:
DUNS: N/A
Number of Employees: N/A
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No