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IMPROVED RFLP ANALYSIS FOR GENETIC LINKAGE MAPPING

Award Information

Agency:
Department of Health and Human Services
Branch:
N/A
Award ID:
8030
Program Year/Program:
1988 / SBIR
Agency Tracking Number:
8030
Solicitation Year:
N/A
Solicitation Topic Code:
N/A
Solicitation Number:
N/A
Small Business Information
Radiation Monitoring Devices, Inc.
44 Hunt Street Watertown, MA 02472-4699
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Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No
 
Phase 1
Fiscal Year: 1988
Title: IMPROVED RFLP ANALYSIS FOR GENETIC LINKAGE MAPPING
Agency: HHS
Contract: N/A
Award Amount: $50,000.00
 

Abstract:

ONE OF THE MOST ACTIVE AND CHALLENGING FIELDS IN MOLECULAR BIOLOGY TODAY IS THE GENETIC STUDY OF EUKARYOTIC SYSTEMS. ONE RAPIDLY GROWING AREA IN THIS FIELD IS THE STUDY OF RESTRICTION FRAGMENT LENGTH POLYMORPHISMS (RFLPS). THIS PROCEDURE USES RADIOLABELED HYBRIDIZATION TECHNIQUES AND AUTORADIOGRAPHY TO CONSTRUCT A HUMAN GENETIC LINKAGE MAP BY IDENTIFYING GENETIC MARKERS ALONG THE GENOME. THE PARTICULAR PART OF THE PROCESS THAT THIS PROPOSAL ADDRESSES IS THE MEASUREMENT OF THE DISTRIBUTION OF RADIOACTIVITY ON THE TRANSFER MEMBRANE. NORMALLY THE AUTORADIOGRAPHS TAKEN OF THIS MEMBRANE REQUIRE AN EXPOSURE OF 16 TO 24 HOURS. FURTHERMORE, UNCERTAINTY IS INTRODUCED IN THE QUANTITATION OF THE RADIOACTIVE BANDS BECAUSE OF THE LIMITED DYNAMIC RANGE OF FILM AND THE RESOLUTION PROBLEMS WHICH ARISE WHEN PHOSPHOROUS-32 PROBES ARE USED. WE PROPOSE TO UTILIZE A NEWLY AVAILABLE, POSITION SENSITIVE NUCLEAR DETECTOR TO GENERATE A REAL TIME IMAGE OF THE DISTRIBUTION OF THE RADIOACTIVITY ON THE TRANSFER MEMBRANE. FURTHERMORE, WE PROPOSE TO PROJECT THIS IMAGE DIRECTLY ONTO A COMPUTER MONITOR FOR VISUALIZATION AND FURTHER DATA ANALYSIS. BY THIS APPROACH, THE RESEARCHER WILL BE ABLE TO AVOID THE DELAYS ASSOCIATED WITH AUTORADIOGRAPHY, AND WILL ALSO BE ABLE TO CARRY OUT THE PROCEDURE WITH A MUCH HIGHER LEVEL OF CONFIDENCE. SUCH AN ABILITY SHOULD SIGNIFICANTLY IMPROVE THE EFFICIENCY OF THE ENTIRE PROCEDURE AND ALLOW MORE RESOURCES TO BE DIRECTED TOWARD THE ANALYTICAL ASPECTS OF THE WORK.

Principal Investigator:


0

Business Contact:

Small Business Information at Submission:

Radiation Monitoring Devices
44 Hunt St Watertown, MA 02172

EIN/Tax ID:
DUNS: N/A
Number of Employees:
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No