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SBIR Phase I: A Protein Probe for High-Throughput SNP Screening

Award Information

Agency:
National Science Foundation
Branch:
N/A
Award ID:
84779
Program Year/Program:
2007 / SBIR
Agency Tracking Number:
0712121
Solicitation Year:
N/A
Solicitation Topic Code:
N/A
Solicitation Number:
N/A
Small Business Information
Radiation Monitoring Devices, Inc.
44 Hunt Street Watertown, MA 02472-4699
View profile »
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No
 
Phase 1
Fiscal Year: 2007
Title: SBIR Phase I: A Protein Probe for High-Throughput SNP Screening
Agency: NSF
Contract: 0712121
Award Amount: $99,994.00
 

Abstract:

This Small Business Small Business Innovation Research (SBIR) Phase I research project aims to develop a reagent that binds specifically to DNA mismatches for use in a microarray-based single nucleotide polymorphism (SNP) assay. Directed evolution will be used to isolate variants of a DNA repair protein with substrate binding properties optimized for this assay. Key challenges will be decreasing the affinity for perfectly complementary oligonucleotides and increasing the spectrum of mismatches bound with sufficient specificity. Because this assay does not rely on subtle differences in hybridization energy for detection of mismatch-containing duplexes, the microarrays require only a small number of probes per SNP (2-4) and are not limited to using short probes. The availability of an inexpensive, flexible, and high throughput SNP detection methodology could lead to better understanding of the genetic components of complex diseases and advance the development of personalized medicine where disease prognosis and treatment is individually tailored.

Principal Investigator:

Daniel G. Hall
DPhil
6176686801
DHall@RMDInc.com

Business Contact:

Daniel G. Hall
DPhil
6176686801
DHall@RMDInc.com
Small Business Information at Submission:

Radiation Monitoring Devices Inc
44 Hunt Street Ste 2100-162 Watertown, MA 02472

EIN/Tax ID: 042546395
DUNS: N/A
Number of Employees:
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No