EFFICIENT CELL-FREE SELECTION OF ANTIBODIES
DESCRIPTION: The objective of this proposal is to develop a novel cell-free
system that would facilitate rapid selection of functional fragments of
monoclonal antibodies. The system descibed is based on well-established
molecular mechanisms. For antibody selection, a cDNA library encoding the
single-chain variable region fragment (scFv) can be used in an in vitro
system to generate a large pool of scFv's that can be selected with target
antigens. The cDNA for the desired scFv can be recovered and used to express
large quantities of recombinant scFv. This procedure is more efficient, less
expensive and technically easier than all existing methods for antibody selection.
It also has the potential to be adapted to high throughput screening systems, or
modified to screen binding proteins other than antibodies.
PROPOSED COMMERCIAL APPLICATION:
The goal for the phase I studies is to test the feasibility of this innovative technology.
Once developed, it may have a major impact on proteomics, cancer diagnosis and drug
Small Business Information at Submission:
Principal Investigator:Jiwu Wang
ALLELE BIOTECH & PHARMACEUTICALS
5310 EASTGATE MALL SAN DIEGO, CA 92121
Number of Employees: