The objective of this proposal is to develop a quantitative ELISA assay for quantitative measurement of anti-RhD antibodies. Specific aims include isolation of soluble serologically active RhD, coating ELISA plates with the extract, enhancing the sensitivity of antibody detection using the… More

DESCRIPTION (provided by applicant): The objective of this proposal is to demonstrate the feasibility of a new approach to improving the performance of bio-molecular imaging methods. A new technology allows one to increase the signal-to-noise ratio, save costly reagents and reduce time of analysis… More

DESCRIPTION (provided by applicant): The objective of this proposal is to demonstrate the feasibility of a new approach to improving the performance of PCR-based methods for HIV detection A new technology allows one to increase the sensitivity, accuracy, dynamic range, signal-to-noise ratio, save… More

DESCRIPTION (provided by applicant): The objective of this proposal is to develop an ultrasound particle agglutination (UPA) method for determination of pathogenic bacteria Escherichia coli O157:H7 and Listeria monocytogenes in body fluids, food and environmental samples. The UPA assay is based on… More

DESCRIPTION (provided by applicant): The objective of this proposal is to demonstrate the feasibility of a novel method for detection of human immunodeficiency virus (HIV) antibody in the body fluids. The method is based on the use of ultrasound to significantly increase the sensitivity and reduce… More

DESCRIPTION (provided by applicant): The objective of the proposed research is to develop an ultrasensitive photochemical amplification method for improving the performance of polymerase-chain reaction (PCR)-based detection of human immunodeficiency virus (HIV). A new technology allows one to… More

DESCRIPTION (provided by applicant): The long-term goal of the proposed research is to develop a novel quantitative particle agglutination technology for improving the performance of detection of human immunodeficiency virus (HIV) antibody in body fluids. The conventional particle agglutination… More

DESCRIPTION (provided by applicant): The long-term goal of the proposed research is to develop sensitive, specific and quantitative enzyme-linked immunosorbent assays (ELISA) for (1) measuring anti-RhD (anti-D) antibodies in patients at risk for Hemolytic Disease of the Newborn (HDN), (2) screening… More

DESCRIPTION (provided by applicant): The objective of this proposal is to develop ultra-sensitive ELISA (Enzyme-Linked ImmunoSorbent Assay) methods for quantification of HIV p24 antigen. At present, the p24 antigen test is relatively insensitive, being abl e to detect only 5- 10 pg/ml. This quantity… More

DESCRIPTION (provided by applicant): The long-term goal of the proposed research is to develop a novel quantitative flow through cell counting method for enumeration of CD4+ T lymphocytes in human blood. CD4 cell counts are needed to stage and monitor HIV- infected patients. In adults, the absolute… More

DESCRIPTION (provided by applicant): The objective of this proposal is to develop ultra-sensitive ELISA (Enzyme-Linked ImmunoSorbent Assay) methods for quantification of HIV p24 antigen. At present, the p24 antigen test is relatively insensitive, being able to detect only 5- 10 pg/ml. This quantity… More

DESCRIPTION (provided by applicant): The long-term goal of the proposed research is to develop a novel quantitative particle agglutination technology for improving the performance of assays for detection of allo-antibodies to red blood cell antigens. Theconventional hemagglutination blood typing… More