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Approach for amplification of molecular signals

Award Information

Agency:
Department of Health and Human Services
Branch:
N/A
Award ID:
76285
Program Year/Program:
2005 / SBIR
Agency Tracking Number:
GM072468
Solicitation Year:
N/A
Solicitation Topic Code:
N/A
Solicitation Number:
N/A
Small Business Information
Alpha Universe LLC
1202 Ann Street Madison, WI 53713-2410
View profile »
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No
 
Phase 1
Fiscal Year: 2005
Title: Approach for amplification of molecular signals
Agency: HHS
Contract: 1R43GM072468-01A1
Award Amount: $164,721.00
 

Abstract:

DESCRIPTION (provided by applicant): Antibody-based detection systems have been used for years in clinical diagnostics and environmental monitoring for the detection and identification of pathogenic agents. Recent advances in the field of low-level antigen detection utilize the DNA polymerase-mediated signal amplification approach that provides a substantial enhancement in detection sensitivity over the conventional ELISA. However, protocols utilizing this approach have not been widely used in diagnostic laboratories because they require rigorous sample preparation, complex reactive components of limited shelf life, expensive hardware and involve a complex detection process. The goal of the current application is to develop an alternative approach for the signal amplification. Bacterial luciferase will be incorporated in the naturally existing cascades of enzymatic reactions to produce an inexpensive and simple assay for the detection of antigens of interest including those of biowarfare agents.

Principal Investigator:

Alexey G. Zdanovsky
6086610861
ALEXEYZD@TDS.NET

Business Contact:

Alexey Zdanovsky
6086610861
ALEXEYZD@TDS.NET
Small Business Information at Submission:

Alpha Universe, LLC
Alpha Universe, Llc 1202 Ann St Madison, WI 53713

EIN/Tax ID: 320102198
DUNS: N/A
Number of Employees: N/A
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No