Development of High-Speed Nucleic Acid Hybridization
Nucleic acid hybridization reactions are at the heart of a number of powerful assays used in molecular biology research and increasingly in diagnostic applications. Hybridization reactions typically require long incubation times, i. e. overnight. Recent work has demonstrated that a ribonucleoprotein and certain cationic detergents can increase hybridization rates several thousand fold such that the reaction will effectively go to completion in a few minutes. The overall goal is to use this technology to develop high-speed nucleic acid hybridization procedures for use in solution and solid phase assays such as Southern blotting. To demonstrate feasibility, we will couple this high-speed hybridization technology to a new streamlined method developed at Ambion for ribonuclease protection assays. We will use nucleic acid probes immobilized to beads to determine optimum conditions for high-speed hybridization. The results from these experiments will then be applied to the development of protocols for high-speed Southern and Northern hybridization procedures. The successful completion of this project will have major impact, both on the research and especially the diagnostic applications of nucleic acid hybridization assays.
Small Business Information at Submission:
Principal Investigator:Matt M. Winkler
2130 Woodward Street #200 Austin, TX 78744
Number of Employees: