USA flag logo/image

An Official Website of the United States Government

Development of High-Speed Nucleic Acid Hybridization

Award Information

Agency:
Department of Health and Human Services
Branch:
N/A
Award ID:
22240
Program Year/Program:
1994 / SBIR
Agency Tracking Number:
22240
Solicitation Year:
N/A
Solicitation Topic Code:
N/A
Solicitation Number:
N/A
Small Business Information
AMBION DIAGNOSTICS, INC.
Asuragen, Inc. 2150 WOODWARD ST AUSTIN, TX -
View profile »
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No
 
Phase 2
Fiscal Year: 1994
Title: Development of High-Speed Nucleic Acid Hybridization
Agency: HHS
Contract: N/A
Award Amount: $491,063.00
 

Abstract:

Nucleic acid hybridization reactions are at the heart of a number of powerful assays used in molecular biology research and increasingly in diagnostic applications. Hybridization reactions typically require long incubation times, i. e. overnight. Recent work has demonstrated that a ribonucleoprotein and certain cationic detergents can increase hybridization rates several thousand fold such that the reaction will effectively go to completion in a few minutes. The overall goal is to use this technology to develop high-speed nucleic acid hybridization procedures for use in solution and solid phase assays such as Southern blotting. To demonstrate feasibility, we will couple this high-speed hybridization technology to a new streamlined method developed at Ambion for ribonuclease protection assays. We will use nucleic acid probes immobilized to beads to determine optimum conditions for high-speed hybridization. The results from these experiments will then be applied to the development of protocols for high-speed Southern and Northern hybridization procedures. The successful completion of this project will have major impact, both on the research and especially the diagnostic applications of nucleic acid hybridization assays.

Principal Investigator:

Matt M. Winkler
5124456979

Business Contact:

Small Business Information at Submission:

Ambion, Inc.
2130 Woodward Street #200 Austin, TX 78744

EIN/Tax ID:
DUNS: N/A
Number of Employees: N/A
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No