ENZYMATIC TOOLS FOR DEGRADING TISSUE AND PRESERVING RNA
DESCRIPTION (provided by applicant):
Intact RNA is an obligate starting material for expression profiling
technologies that can link specific molecular events with disease phenotypes.
RNA isolations from tissue currently require invasive mechanical force to
disrupt the cellular architecture. Such mechanical approaches are tedious and
potentially inefficient. They also expose the operator to possible biohazards.
As a result, the development of a "closed system" for RNA isolation is needed
to enable the high-throughput recovery of RNA from tissues such as biopsies
using a safeguarded platform that can process dozens or hundreds of tissues at
once. We proposed to achieve this goal through the use of "MELT"
(Multi-Enzymatic Liquefaction of Tissue) technology. This technology will
harness the degrading power of enzymes that have evolved together as a
biological tool set for the decomposition of complex food sources. We refer to
this tool set as an "enzyme community." Since some enzyme communities (e.g.,
the secreted enzymes of bacterial pathogens) mimic the spectrum of activities
needed to digest tissue, these collections are an ideal repository for
tissue-degrading enzymes. We propose to characterize and blend enzyme
activities to create a MELT reagent that can rapidly degrade tissues, "freeze"
mRNA expression profiles, and stabilize the RNA for downstream analyses.
Small Business Information at Submission:
AMBION, INC. 2130 WOODWARD ST, #200 AUSTIN, TX 78746
Number of Employees: