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Genomic siRNA Libraries as Tools for Pseudo-Genetics

Award Information

Agency:
Department of Health and Human Services
Branch:
N/A
Award ID:
65613
Program Year/Program:
2003 / SBIR
Agency Tracking Number:
HG003270
Solicitation Year:
N/A
Solicitation Topic Code:
N/A
Solicitation Number:
N/A
Small Business Information
AMBION DIAGNOSTICS, INC.
Asuragen, Inc. 2150 WOODWARD ST AUSTIN, TX -
View profile »
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No
 
Phase 1
Fiscal Year: 2003
Title: Genomic siRNA Libraries as Tools for Pseudo-Genetics
Agency: HHS
Contract: 1R43HG003270-01
Award Amount: $254,451.00
 

Abstract:

DESCRIPTION (provided by applicant): The proposed research will enable mammalian cells to be used in mutation/selection experiments to identify genes involved in cellular processes. The objective of the proposal is to develop procedures for preparing siRNA libraries using genomic DNA and cDNA samples as the source material for siRNA templates. Libraries of siRNA vectors could comprise 1,000,000,000 to 1,000,000,000,000 unique siRNA expression vectors, making it possible to cover entire mammalian genomes with overlapping siRNAs. Mammalian cell populations transfected or transduced with siRNA vector libraries will be placed under selection to create cell populations expressing a desirable phenotype. The siRNA vector(s) integrated in the genomes of the selected cells will be amplified, cloned, and sequenced to reveal the siRNA template sequence. The siRNA sequences will be used to identify the genes whose down-regulation create the phenotype that was selected. The siRNA vector libraries should enable mutation/selection experiments to be used to correlate gene function and identity in mammalian cells.

Principal Investigator:

David M. Brown
5126510200
DBROWN@AMBION.COM

Business Contact:

Rigo Vallejo
5126510200
RVALLEJO@AMBION.COM
Small Business Information at Submission:

AMBION, INC.
2130 WOODWARD STREET AUSTIN, TX 78744

EIN/Tax ID: 742515119
DUNS: N/A
Number of Employees: N/A
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No