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A NEW HAIRY CELL LEUKEMIA CELL LINE

Award Information

Agency:
Department of Health and Human Services
Branch:
N/A
Award ID:
19090
Program Year/Program:
1992 / SBIR
Agency Tracking Number:
19090
Solicitation Year:
N/A
Solicitation Topic Code:
N/A
Solicitation Number:
N/A
Small Business Information
AMERICAN LABORATORY SUPPLIES
2000 N 14TH ST, STE 530 ARLINGTON, VA 22201
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Woman-Owned: Yes
Minority-Owned: Yes
HUBZone-Owned: No
 
Phase 1
Fiscal Year: 1992
Title: A NEW HAIRY CELL LEUKEMIA CELL LINE
Agency: HHS
Contract: N/A
Award Amount: $49,875.00
 

Abstract:

A NEW CELL LINE, DESIGNATED "HDS", WAS ESTABLISHED IN A SUSPENSION CULTURE DERIVED FROM THE PERIPHERAL BLOOD OF A PATIENT WITH HAIRY CELL LEUKEMIA (HCL), AND THE PERSON HAS DEVELOPED CLINICAL RESISTANCE TO ALPHA-INTERFERON THERAPY. THE PATIENT EXHIBITED A CLINICAL PICTURE CHARACTERISTIC OF HCL, INCLUDING SPLENOMEGALY, CYTOPENIAS, AND TARTRATE-RESISTANT ACID PHOSPHATASE (TRAP)-POSITIVE "HAIRY" CELLS IN BLOOD AND MARROW. ULTRA-STRUCTURAL ANALYSIS OF THE CULTURED CELLS, AT THE SINGLE CELL LEVEL, REVEALED A DISPLAY OF MICROVILLI, MIXED WITH RUFFLES ON THE CELL SURFACE IN A CLASSICAL HAIRY CELL PATTERN. THE ESTABLISHED CELL LINE HAS THE PHENOTYPE OF A B-TYPE CELL AS JUDGED BY CYTOCHEMISTRY AND IMMUNOLABELING. HDS CELLS HAVE HLA-DR AND CD19 SURFACE ANTIGENS, BUT SHOWN NEGATIVE RESULTS FOR EARLY B(CD10) AND EARLY T (CD2, CD3) CELL MARKERS. THESE CELLS ARE NEGATIVE FOR HCL MARKERS SUCH AS CD11C, CD22, AND CD25. HDS CELLS ARE ALSO NEGATIVE FOR T-CELLS, MYELOCYTIC, AND MONOCYTIC MARKERS. IN PHASE I AND II, STUDIES ARE PLANNED TO DETERMINE THE EXACT DIFFERENTIATION STAGE OF THE HDS CELLS, AND TO DEVELOP A NOVEL SUBCLASS OF B-TYPE HCL CELLS, HDS CELLS FOR CLINICAL ASSAYS, DIAGNOSIS, DETECTION AND TREATMENT. THIS WORK IS IMPORTANT IN CLINICAL HEMATOLOGY RESEARCH DIAGNOSIS.

Principal Investigator:

Shi-hua Wu
7035583400

Business Contact:

Small Business Information at Submission:

Cp Li Biomedical Research
2000 North 14th Street Suite 740 Arlington, VA 22201

EIN/Tax ID:
DUNS: N/A
Number of Employees: N/A
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No