An electrospray (es) ion source will be interfaced to a time-of-flight (tof) mass spectrometer incorporating a pseudo-random-chopping technique. the tof mass spectrometerwill allow rapid scan rates and high-mass analysis for multiple and singly charged ions delivered from the es ion source. using a… More

This research will evaluate a novel method of transporting ions from an external source, that can be at any desired pressure, into a vacuum system containing a mass analyzer. of particular interest are "soft" sources that produce intact ions from large, complex, and fragile species of biochemical… More

An electrospray ionization (espi) source will be interfaced with an ion mobility analyzer (ima), and performance characteristics will be studied. to establish the potentialfor commercialization of this system, detection sensitivity limits will be determined for a range of sample types. in addition,… More

Recent developments have shown that electrospray ionization can produce intact ions from large, complex, and fragile species with great efficiency and extensive multiple charging. subnanomole samples of polypeptides and small proteins in the mass range of 25 kilodaltons have been analyzed. because… More

An electrospray-tandem-time-of-flight mass spectrometer (espi-ttof-ms) has been designed and built for the purpose of obtaining both molecular weight and structural information of biological molecules. the objective of this project is t address the issue of data capture and treatment from such an… More

The overall objective of this research is to continue to improve electrospray ionization mass spectrometry (espi-ms) for the analysis of biological macromolecules. as a method capable of delivering extremely large molecules into the gas phase as intact desolvated molecular ionic species from their… More

Electrospray ionization combined with mass spectrometry has evolved as a powerful technique for the analysis of a wide range of compounds of interest to the field of molecular biology. the research aims to combine electrospray ionization with a mechanical vibration assist. ultrasonic nebulizers… More

In this study, we will use electrospray to form monodisperse droplets of controlled size from drug containing aqueous solutions. the accurate control of droplet sizes and size distributions would increase the precision by which aerosol delivered drugs could be targeted to various parts of the… More

A laser-driven Vacuum Ultraviolet (VUV) photoionization source is proposed as a general purpose, high efficiency device for creating ionized species of biological molecules of several hundred amu or less in the gaseous phase. The high energy of the monochromatic VUV photons produced allows single… More

A specialized mass spectrometer is proposed as a universal detector for liquid chromatographyapplications. This device will be highly suited to the analysis of organic compounds in complex fluidsand will allow biomedical researchers to both identify and quantitate various species of interest… More

A novel combination of techniques is proposed as a mass detector for biological compounds.A quadruple ion trap is used as a storage device for ions introduced from an Electrospray ionizationsource, thereby providing 100% duty cycle, while a reflection time-of-flight mass spectrometer is usedfor the… More

We will develop a high speed data system which uses a time-of-flight mass spectrometer (TOF-MS) asmass separation device and species detector. This data system will take advantage of the temporal spmass spectrometry to rapidly compute and display either liquid chromatography (LC) or capillary… More

DESCRIPTION (provided by applicant): Many areas of biomedical research have benefited greatly from recent progress in mass spectrometry instrumentation and techniques. Time-of-flight mass spectrometry, in particular, has emerged as an important tool in molecular biology and biomedical research and… More

DESCRIPTION (provided by applicant): Mass spectrometry has become an essential analytical tool for clinical diagnostics, drug discovery, and similar biomedical research areas, due in large part to its ability to elucidate the structure of biological molecules. Such information is commonly derived… More