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Nonradioactive Receptor Binding Assays

Award Information

Agency:
Department of Health and Human Services
Branch:
N/A
Award ID:
29586
Program Year/Program:
1995 / SBIR
Agency Tracking Number:
29586
Solicitation Year:
N/A
Solicitation Topic Code:
N/A
Solicitation Number:
N/A
Small Business Information
ANALYTICAL BIOLOGICAL SERVICES, INC.
ANALYTICAL BIOLOGICAL SERVICES 701-4 CORNELL BUSINESS PARK WILMINGTON, DE 19801
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Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No
 
Phase 1
Fiscal Year: 1995
Title: Nonradioactive Receptor Binding Assays
Agency: HHS
Contract: 1 R43 MH53461-01,
Award Amount: $76,286.00
 

Abstract:

The use of biotin labeled ligands in receptor binding assays will be used as a substitute for radioHigh affinity biotinylated ligands are used in immunochemistry studies but are not generally employebinding experiments. Biotinylated ligands also have high affinities for the proteins avidin and streproteins can be coupled with various enzymes which catalyze spectrophotometric, fluorometric, or chedetection in microliter plates. Using standard receptor binding protocols, biotinylated ligands canappropriate receptor and possible competitors for the receptor. Free or receptor bound biotinylatedby binding to the avidin or st reptavidin enzyme conjugates. This mechanism makes it possible for onbiotinylated ligand to be responsible for the generation of many molecules of product, providing aninitial signal. Several strategies for selected receptor binding assays, utilizing biotinylated ligaassay format assays will be examined. The knowledge gained in these investigations should aid in theadditional high throughput nonradioactive receptor binding assays.

Principal Investigator:

Charles Saller
30254

Business Contact:

Small Business Information at Submission:

Analytical Biological
Cornell Business Park 701-4 Wilmington, DE 19801

EIN/Tax ID:
DUNS: N/A
Number of Employees: N/A
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No