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Detergents for Dissociation of Lipid Rafts

Award Information

Agency:
Department of Health and Human Services
Branch:
N/A
Award ID:
66486
Program Year/Program:
2003 / SBIR
Agency Tracking Number:
GM069021
Solicitation Year:
N/A
Solicitation Topic Code:
N/A
Solicitation Number:
N/A
Small Business Information
ANATRACE, INC.
ANATRACE, INC. 26111 Miles Rd Cleveland, OH 44128 5933
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Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No
 
Phase 1
Fiscal Year: 2003
Title: Detergents for Dissociation of Lipid Rafts
Agency: HHS
Contract: 1R43GM069021-01
Award Amount: $77,436.00
 

Abstract:

DESCRIPTION (provided by applicant): Glycosphingolipids and cholesterol form associations with important signaling proteins, within biological membranes forming what are called "rafts". These rafts are characterized by their insolubility even in the presence of many detergents used in membrane chemistry to isolate proteins from the lipid components of membranes. Some of the most effective detergents for disrupting membrane rafts are derived from nature, e.g. the saponins and digitonin. Others are based on derivatized cyclodextrins such as methyl-beta-cyclodextrin (MBCD). None of the above are perfectly satisfactory. The saponins are of uncertain composition, digitonin is unstable in solution and highly toxic, and MBCD is expensive. A component of all rafts is cholesterol. This may account for the propensity of the steroid related structures of the saponins and digitonin to function as solubilizing agents. We propose to synthesize and test new detergents that incorporate cholesterol as the hydrophobic portion of the molecule. We will synthesize these new detergent molecules from inexpensive starting materials. The product materials will be water soluble and, since based on cholesterol, are expected to be non-toxic.

Principal Investigator:

Melvin H. Keyes
4198913030
ANATRAC434@AOL.COM

Business Contact:

Barry Watson
4198913030
BWATSON@GLASSCITY.NET
Small Business Information at Submission:

ANATRACE, INC.
ANATRACE, INC. 1280 DUSSEL DR MAUMEE, OH 43537

EIN/Tax ID: 341458982
DUNS: N/A
Number of Employees: N/A
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No